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Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

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ELISA: Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) [NBP2-75054] - Samples were spiked with high concentrations of Rat Serpin A6/Cortisol Binding Globulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) [NBP2-75054] - Samples were spiked with high concentrations of Rat Serpin A6/Cortisol Binding Globulin and diluted with Reference Standard & Sample ...read more
ELISA: Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) [NBP2-75054] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
6.25 - 400 ng/mL
Sensitivity
3.75 ng/mL

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Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CBG in samples. No significant cross-reactivity or interference between Rat CBG and analogues was observed.
Standard Curve Range
6.25 - 400 ng/mL
Sensitivity
3.75 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.39%
Intra-Assay
CV% < 5.25%
Spike Recovery
89-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINA6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

  • antitrypsin), member 6
  • CBG
  • CBGcorticosteroid-binding globulin
  • corticosteroid binding globulin
  • member 6
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A6
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • transcortin

Background

Corticol binding globulin (CBG or transcotin) binds with high affinity to cortisol in plasma. Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol SERPINA6