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SEC61A Antibody

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Western Blot: SEC61A Antibody [NB100-768] - Analysis of SEC61A in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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SEC61A Antibody Summary

Immunogen
Peptide with sequence C-KEQSEVGSMGALLF corresponding to C-Terminus according to NP_037468.1.
Epitope
C-KEQSEVGSMGALLF
Specificity
This antibody is designed to detect SEC61A1 and should not detect SEC61A2, but this has not been determined experimentally.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEC61A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human brain (Cerebelum) lysates (calculated MW of 52.3 kDa band according to NP_037468.1).
Publications
Read Publication using NB100-768.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SEC61A Antibody

  • HSEC61
  • protein transport protein SEC61 alpha subunit
  • protein transport protein Sec61 subunit alpha isoform 1
  • protein transport protein Sec61 subunit alpha
  • Sec61 alpha 1 subunit (S. cerevisiae)
  • Sec61 alpha-1
  • sec61 homolog
  • SEC61
  • SEC61A

Background

SEC61A1 belongs to the SECY/SEC61 alpha family. It has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.The SEC61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. SEC61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. SEC61 alpha is a polytopic protein that has ten membrane-spanning loops. SEC61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SEC61A Antibody (NB100-768)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SEC61A Products

Array NB100-768

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Bioinformatics

Gene Symbol SEC61A1
Entrez
Uniprot