![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 expression in transfected 293T cell line by MCFD2 monoclonal antibody (M01), clone 3A5-G4.Lane 1: MCFD2 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0012.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 expression in transfected 293T cell line by MCFD2 monoclonal antibody (M01), clone 3A5-G4.Lane 1: MCFD2 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 3A5-G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | MCFD2 (AAH40357, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMRSLLRTPFLCGLLWAFCAPGARAEEPAASFSQPGSMGLDKNTVHDQEHIMEHLEGVINKPEAEMSPQELQLHYFKMHDYDGNNLLDGLELSTAITHVHKEEGSEQAPLMSEDELINIIDGVLRDDDKNNDGYIDYAEFAKSLQ |
| Specificity | MCFD2 - multiple coagulation factor deficiency 2 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCFD2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for Western Blot. It has also been used for immunohistochemistry (paraffin), ELISA and RNAi validation. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SDNSF/MCFD2 Antibody (H00090411-M01)Find related products by research area.
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![Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of monoclonal antibody to MCFD2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SDNSF-MCFD2-Antibody-3A5-G4-Immunohistochemistry-Paraffin-H00090411-M01-img0008.jpg "Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of monoclonal antibody to MCFD2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.")
![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 over-expressed 293 cell line, cotransfected with MCFD2 Validated Chimera RNAi ( Cat # H00090411-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCFD2 monoclonal antibody (M01), clone 3A5-G4 (Cat # H00090411-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0009.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 over-expressed 293 cell line, cotransfected with MCFD2 Validated Chimera RNAi ( Cat # H00090411-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCFD2 monoclonal antibody (M01), clone 3A5-G4 (Cat # H00090411-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Detection limit for recombinant GST tagged MCFD2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SDNSF-MCFD2-Antibody-3A5-G4-Sandwich-ELISA-H00090411-M01-img0011.jpg "Sandwich ELISA: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Detection limit for recombinant GST tagged MCFD2 is 0.1 ng/ml as a capture antibody.")
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