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Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

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ELISA: SCF/c-kit Ligand ELISA Kit [HRP] [KA1022] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum
Standard Curve Range
0.156 to 40 ng/mL
Sensitivity
0.09 ng/mL

Order Details

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Human SCF/c-kit Ligand ELISA Kit (Colorimetric) Summary

Description
KITLG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human KITLG in plasma, serum, and cell culture supernatants.
Standard Curve Range
0.156 to 40 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.20%
Intra-Assay
CV% < 4.50%
Spike Recovery
96%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KITLG

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using
KA1022 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated SCF Antibody (100x): A 100-fold concentrated biotinylated polyclonalantibody against SCF.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  4. Human SCF Microplate: A polystyrene microplate coated with a polyclonal antibodyagainst human SCF.
  5. Human SCF Standard: Human SCF in a buffered protein base (lyophilized).
  6. Sealing Tapes: Each kit contains pre-cut, pressure-sensitive sealing tapes that can becut to fit the format of the individual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for SCF/c-kit Ligand ELISA Kit (KA1022)(4)

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Bioinformatics

Gene Symbol KITLG