Human SCF/c-kit Ligand ELISA Kit (Colorimetric) Summary
Description
KITLG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human KITLG in plasma, serum, and cell culture supernatants.
Standard Curve Range
0.156 to 40 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.20%
Intra-Assay
CV% < 4.50%
Spike Recovery
96%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KITLG
Applications/Dilutions
Dilutions
ELISA
Quantification
Publications
Read Publications using KA1022 in the following applications:
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated SCF Antibody (100x): A 100-fold concentrated biotinylated polyclonalantibody against SCF.
Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
Human SCF Microplate: A polystyrene microplate coated with a polyclonal antibodyagainst human SCF.
Human SCF Standard: Human SCF in a buffered protein base (lyophilized).
Sealing Tapes: Each kit contains pre-cut, pressure-sensitive sealing tapes that can becut to fit the format of the individual assay.
Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)
c-kit Ligand
DCUA
DFNA69
DKFZp686F2250
familial progressive hyperpigmentation 2
FPH2
FPHH
KIT ligand
Kitl
KITLG
KL-1
Mast cell growth factor
MGF
MGFSHEP7
SCF
SCFStem cell factor
SFc-Kit ligand
SHEP7
SLF
steel factor
Background
Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.