Applications | WB, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Specificity | Detects human SCF/c-kit Ligand in ELISAs and Western blots. In sandwich immunoassays, approximately 55% cross-reactivity with recombinant feline SCF is observed, 20% cross-reactivity with recombinant canine SCF is observed, and less than 0.3% cross-reactivity with recombinant mouse SCF is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | KITLG |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Application Notes | ELISA Capture: Human SCF/c-kit Ligand Antibody (Catalog # MAB655) ELISA Detection: Human SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF255) Standard: Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) |
Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75%-83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).
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Gene Symbol | KITLG |