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Recombinant Human SAT1 His Protein

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SDS-Page: Recombinant Human SAT1 Protein [NBP1-30198] - Diamine N-acetyltransferase 1, 22.1 kDa (191aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human SAT1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-171 of Human SAT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SAT1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human SAT1 His Protein

  • DC21
  • diamine acetyltransferase 1
  • diamine N-acetyltransferase 1
  • EC 2.3.1.57
  • KFSD
  • Polyamine N-acetyltransferase 1
  • Putrescine acetyltransferase
  • SAT
  • Spermidine/spermine N(1)-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase alpha
  • spermidine/spermine N1-acetyltransferase
  • SSAT-1
  • SSATKFSDX

Background

Diamine N-acetyltransferase 1(or Spermidine/spermine-N1-acetyltransferase, SSAT1), as known as SAT-1, is a polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This protein plays an important role in polyamine homoeostasis, since acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. A variety of other effects of increased SAT-1 activity include death of pancreatic cells, blockage of regenerative tissue growth, behavioral changes, keratosis follicularis spinulosa decalvans(KFSD), and hair loss. Recombinant human SAT-1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for SAT1 Recombinant Protein (NBP1-30198) (0)

There are no publications for SAT1 Recombinant Protein (NBP1-30198).
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FAQs for SAT1 Recombinant Protein (NBP1-30198). (Showing 1 - 2 of 2 FAQ).

  1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
    • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).
  2. Have you ever tested the specific activity of the recombnant enzyme NBP1-30198? Could it be used to acetylate substrate Spermine?
    • We have not tested the activity of NBP1-30198. This protein has only been validated as a positive control for SDS-PAGE and WB, so we would not recommend using it for any experiment requiring activity or functionality. I have checked our inventory to see if we have any SAT1 proteins that would be suitable for acetylating Spermine, but unfortunately we do not have any other SAT1 proteins.

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Bioinformatics

Gene Symbol SAT1