SARS Nucleocapsid Protein Antibody Pack Summary
| Description |
This pack contains 1 vial each of: NBP2-44205-25ug (25 ug), NB100-56576SS (25 ug), NB100-56683SS (25 ug). NBP2-44205 is manufactured exclusively by Rockland Immunochemicals. |
| Immunogen |
See individual datasheets. |
| Clonality |
Polyclonal |
| Gene |
N |
Applications/Dilutions
| Dilutions |
- Dual RNAscope ISH-IHC
- ELISA
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry-Paraffin
- Western Blot
|
| Application Notes |
See individual datasheets of components for their validated applications |
Reactivity Notes
See individual datasheets of components for their validated species
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
NB100-56576 and NB100-56683: PBS, 0.05% Sodium Azide. NBP2-44205: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide. |
Kit Components
Alternate Names for SARS Nucleocapsid Protein Antibody Pack
Background
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
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Additional SARS Nucleocapsid Protein Products
Blogs on SARS Nucleocapsid Protein
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0003.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP3-05695-img0001.jpg "Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].")
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0004.jpg "Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.")
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody[NBP2-44205] at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0006.jpg "Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody[NBP2-44205] at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0005.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628 SARS Nucleocapsid Protein Antibody [NB100-56576]](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0002.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628 SARS Nucleocapsid Protein Antibody [NB100-56576]")