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![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0005.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56576-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with SARS-CoV (MOI = 0.1) for 24, 48, and 72 hrs before being fixed with 4% paraformaldehyde for monitoring the morphological changes of infected cells, as visualized by the expression of SARS-CoV NP protein (red) by using the standard IHC (F). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008729) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NB100-56576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0007.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0002.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0004.jpg "Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56683-img0005.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56683-img0002.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0003.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP3-05695-img0001.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0004.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody[NBP2-44205] at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Western-Blot-NBP3-05695-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0005.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628 SARS Nucleocapsid Protein Antibody [NB100-56576]](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Pack-Immunohistochemistry-NBP3-05695-img0002.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-00510] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NBP3-00510) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Western-Blot-NBP3-00510-img0002.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-00510] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NBP3-00510) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.")
![Simple Western: SARS-CoV-2 Nucleocapsid Antibody [NBP3-00510] - Simple Western lane view shows recombinant SARS-CoV-2 Spike S1 RBD (receptor binding domain) protein (negative sample), recombinant SARS-CoV-2 Spike S1 subunit protein (negative sample), recombinant SARS-CoV-2 Spike S1/S2 subunit protein (negative sample), and recombinant SARS-CoV-2 Nucleocapsid protein, loaded at 0.2 mg/mL. Specific bands were detected for SARS-CoV-2 Nucleocapsid at approximately 60 kDa (as indicated) using 20 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NBP3-00510). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Western-Blot-NBP3-00510-img0001.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56049-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-004-Western-Blot-NBP3-12734-img0003.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition")
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Analysis of Nucleocapsid overexpresssed Hek293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a AlexaFluor®488-conjugated second step antibody.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-004-Immunocytochemistry-Immunofluorescence-NBP3-12734-img0002.jpg)
![Flow Cytometry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-004-Flow-Cytometry-NBP3-12734-img0004.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein Lane B: SARS-CoV NP Protein Lane C: SARS-CoV-2 (2019-nCoV) NP Protein Lane D: SARS-CoV-2 (2019-nCoV) NP Protein SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-004-Western-Blot-NBP3-12734-img0001.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0004.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44205-img0003.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NBP2-44205-img0005.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: SARS CoV Nucleocapsid (N) Protein [20 ng]. Primary Antibody: Anti-SARS CoV Nucleocapsid (N) Protein Antibody at 1.0 ug/mL overnight at 2-8 degrees C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40000 for 30 mins at RT. Predicted MW: ~46 kDa. Observed MW: ~48 kDa.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0001.jpg)
![ELISA: SARS Nucleocapsid Protein Antibody [NBP2-44205] - ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5ug/mL of SARS CoV-1 [Red Line], SARS CoV-2 Nc [Green Line], MERS CoV N [Blue Line]. The starting dilution of antibody was 2 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1N IC50: 11ng/mL, SARS CoV-2N IC50: 15 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-ELISA-NBP2-44205-img0006.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (3851) [NBP3-05706] - Validation with Human Recombinant Protein Loading: 50 ng per lane of human nucleocapsid recombinant protein. SARS-CoV-2 nucleocapsid. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3851-Western-Blot-NBP3-05706-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (3851) [NBP3-05706] - Validation with Human Recombinant Protein Loading: 50 ng per lane of human nucleocapsid recombinant protein. SARS-CoV-2 nucleocapsid. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL")
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (3851) [NBP3-05706] - Antibodies: SARS-CoV-2 Nucleocapsid antibody,NBP3-05706 . An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat antimouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 2000 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3851-ELISA-NBP3-05706-img0003.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/ml. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/ml, showing very strong and specific binding to SARS-CoV-2 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-84C4a-ELISA-NBP3-07041-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/ml. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/ml, showing very strong and specific binding to SARS-CoV-2 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid specific rabbit monoclonal antibodies: capture antibody and biotin-conjugated a detection antibody. SARS-CoV-2 Nucleocapsid was serially diluted 1:2 starting at 25 ng/ml. The capture antibody and biotin-conjugated a detection antibodies detected SARS-CoV-2 Nucleocapsid antigen at very high sensitivity as low as 100 pg/ml (100 pg).](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-84C4a-Sandwich-ELISA-NBP3-07041-img0002.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a as a capture antibody and75G5a-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detectedCOVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-2711202420414515.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (84C4a), CoV2NP-84C4.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-271120241920156.jpg)
![Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-2711202420175139.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a wasserially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-2711202419553055.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a as a capture antibody and75G5a-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detectedCOVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-2711202419293838.jpg)
![Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-271120242013256.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (84C4a), CoV2NP-84C4.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-2711202419432222.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (84C4a) [NBP3-07041] - Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a wasserially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.](http://images.novusbio.com/fullsize/nbp3-07041_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-84c4a-271120242092038.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56050-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-05730] - Validation of SARS-CoV-2 Nucleocapsid Antibody, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Western-Blot-NBP3-05730-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-05730] - Validation of SARS-CoV-2 Nucleocapsid Antibody, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.")
![N/A: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) [NBP3-07043] - Exp1293 cells were transfected with expression plasmid coding for SARS-CoV-2 Nucleocapsid and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supernatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-1C7C7-N-A-NBP3-07043-img0001.jpg "N/A: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) [NBP3-07043] - Exp1293 cells were transfected with expression plasmid coding for SARS-CoV-2 Nucleocapsid and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supernatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.](http://images.novusbio.com/fullsize/nbp3-07043_mouse-sars-cov-2-nucleocapsid-mab-1c7c7-azide-bsa-free-271120241937776.jpg)
![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.](http://images.novusbio.com/fullsize/nbp3-07043_mouse-sars-cov-2-nucleocapsid-mab-1c7c7-azide-bsa-free-2711202420312935.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.](http://images.novusbio.com/fullsize/nbp3-07043_mouse-sars-cov-2-nucleocapsid-mab-1c7c7-azide-bsa-free-2711202419475817.jpg)
![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.](http://images.novusbio.com/fullsize/nbp3-07043_mouse-sars-cov-2-nucleocapsid-mab-1c7c7-azide-bsa-free-2711202420441144.jpg)