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SAP155 Antibody

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Western Blot: SAP155 Antibody [NBP3-35603] - Western blot analysis of various lysates using SAP155 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Western Blot: SAP155 Antibody [NBP3-35603] - Western blot analysis of various lysates using SAP155 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of C6 using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of L929 using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of U-2OS using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SAP155 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SAP155 (NP_001005526.1).

Sequence:
MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGFYSAA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for SAP155 Antibody

  • Hsh155
  • pre-mRNA processing 10
  • pre-mRNA splicing factor SF3b, 155 kDa subunit
  • Pre-mRNA-splicing factor SF3b 155 kDa subunit
  • Prp10
  • PRPF10
  • SAP 155
  • SAP155splicing factor 3b, subunit 1, 155kD
  • SF3B155
  • SF3b155PRP10
  • spliceosome associated protein 155
  • Spliceosome-associated protein 155
  • splicing factor 3B subunit 1
  • splicing factor 3b, subunit 1, 155kDa

Background

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SAP155 Antibody
NBP3-35603
SAP155 Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SF3B1