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SAP155 Antibody

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Western Blot: SAP155 Antibody [NBP1-57192] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml
Western Blot: SAP155 Antibody [NBP1-57192] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml
Immunohistochemistry: SAP155 Antibody [NBP1-57192] - Human cardiac cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.
Immunohistochemistry-Paraffin: SAP155 Antibody [NBP1-57192] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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SAP155 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to SF3B1 (splicing factor 3b, subunit 1, 155kDa) The peptide sequence was selected from the middle region of SF3B1. Peptide sequence LLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADG. The peptide sequence for this immunogen was taken from within the described region.
Specificity
This product is specific to Subunit or Isoform: 1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SAP155 Antibody

  • Hsh155
  • pre-mRNA processing 10
  • pre-mRNA splicing factor SF3b, 155 kDa subunit
  • Pre-mRNA-splicing factor SF3b 155 kDa subunit
  • Prp10
  • PRPF10
  • SAP 155
  • SAP155splicing factor 3b, subunit 1, 155kD
  • SF3B155
  • SF3b155PRP10
  • spliceosome associated protein 155
  • Spliceosome-associated protein 155
  • splicing factor 3B subunit 1
  • splicing factor 3b, subunit 1, 155kDa

Background

SF3B1 is the subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SAP155 Antibody
NBP1-57192
SAP155 Antibody
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol SF3B1