SAM68 Antibody (1A4)

Images

 
Western Blot: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of KHDRBS1 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of monoclonal antibody to KHDRBS1 on A-431 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Western Blot: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody (M03), clone 1A4. Lane 1: KHDRBS1 transfected lysatE (41 KDa). Lane 2: ...read more
ELISA: SAM68 Antibody (1A4) [H00010657-M03] - Detection limit for recombinant GST tagged KHDRBS1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SAM68 Antibody (1A4) Summary

Immunogen
KHDRBS1 (AAH00717, 176 a.a. ~ 275 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLN
Specificity
KHDRBS1 (1A4)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KHDRBS1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SAM68 Antibody (1A4)

  • FLJ34027
  • GAP-associated tyrosine phosphoprotein p62
  • KH domain containing, RNA binding, signal transduction associated 1
  • KH domain-containing, RNA-binding, signal transduction-associated protein 1
  • KHDRBS1
  • p21 Ras GTPase-activating protein-associated p62
  • p62
  • p68
  • SAM68
  • Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68)
  • Src-associated in mitosis 68 kDa protein

Background

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KHDRBS1
Entrez
OMIM
Uniprot