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RPS14 Antibody

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Western Blot: RPS14 Antibody [NBP1-57365] - Jurkat, Antibody Dilution: 1.0 ug/ml RPS14 is supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: RPS14 Antibody [NBP1-57365] - Titration: 2.5ug/ml, Positive Control: HepG2 cell lysate.
Western Blot: RPS14 Antibody [NBP1-57365] - Analysis of 721_B cell lysate. Antibody Dilution: 1.0 ug/ml RPS14 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
Western Blot: RPS14 Antibody [NBP1-57365] - Hela, Antibody Dilution: 1.0 ug/ml RPS14 is supported by BioGPS gene expression data to be expressed in HeLa.
Western Blot: RPS14 Antibody [NBP1-57365] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.
Western Blot: RPS14 Antibody [NBP1-57365] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: RPS14 Antibody [NBP1-57365] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Western Blot: RPS14 Antibody [NBP1-57365] - MCF7, Antibody Dilution: 1.0 ug/ml RPS14 is supported by BioGPS gene expression data to be expressed in MCF7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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RPS14 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to RPS14 (ribosomal protein S14) The peptide sequence was selected from the middle region of RPS14. Peptide sequence GNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for RPS14 Antibody

  • emetine resistance
  • EMTB40S ribosomal protein S14
  • ribosomal protein S14

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS14 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RPS14
Uniprot