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ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)]

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Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human ROR gamma/RORC/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6109C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Human IL-23 (Catalog # 1290-IL) were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
600214
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP (Peridinin-chlorophyll Protein Complex)

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
MAB6109
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6109
Carrier Free
MAB6109
Carrier Free
MAB6109-SP
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ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

Immunogen
E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln100
Accession # P51449-2
Specificity
Detects human and mouse ROR gamma /RORC/NR1F3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RORC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC6109C in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)]

  • MGC129539
  • NR1F3
  • Nuclear receptor ROR gamma
  • Nuclear receptor ROR-gamma
  • Nuclear receptor RZR gamma
  • Nuclear receptor RZR-gamma
  • Nuclear receptor subfamily 1 group F member 3
  • RAR-Related Orphan Nuclear Receptor Variant 2
  • RAR-related orphan receptor C
  • RAR-related orphan receptor gamma
  • retinoic acid-binding receptor gamma
  • retinoid-related orphan receptor gamma
  • Retinoid-related orphan receptor-gamma
  • ROR gamma
  • RORC
  • RORG
  • RORGMGC129539
  • RZRG
  • RZRGRZR-GAMMA
  • TOR

Background

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ROR gamma/RORC/NR1F3 Antibody (600214) [PerCP (Peridinin-chlorophyll Protein Complex)]
Species: Hu
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Publications for ROR gamma/RORC/NR1F3 Antibody (IC6109C)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Reviews for ROR gamma/RORC/NR1F3 Antibody (IC6109C) (0)

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FAQs for ROR gamma/RORC/NR1F3 Antibody (IC6109C). (Showing 1 - 3 of 3 FAQ).

  1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
    • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
  2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
    • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
  3. What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?
    • A sequence from Met1 - Gln100.

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Bioinformatics

Gene Symbol RORC
Uniprot