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Rit1 Antibody (456201)

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Western blot shows lysates of human brain tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rit1 Monoclonal Antibody (Catalog # MAB4697) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
456201
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Rit1 Antibody (456201) Summary

Immunogen
E. coli-derived recombinant human Rit1
Met1-Thr219
Accession # Q92963
Specificity
Detects human Rit1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RIT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rit1 Antibody (456201)

  • GTP-binding protein Rit1
  • GTP-binding protein Roc1
  • MGC125864
  • MGC125865
  • Ras-like protein expressed in many tissues
  • Ras-like without CAAX 1
  • Ras-like without CAAX protein 1
  • RIBB
  • RIBBRIT
  • Ric-like, expressed in many tissues
  • RIT
  • Rit1
  • ROC1
  • ROC1Ric (Drosophila)-like, expressed in many tissues

Background

Rit1 is a member of the Ras-related small GTPase superfamily. The 219 amino acid, 25 kDa human Rit1 is a cytoplasmic cell membrane-associated protein that is expressed in most tissues. It is widely expressed in the developing and adult nervous systems and participates in NGF-induced axon outgrowth. Rit1 forms complexes with other small GTPases, such as cdc42 and Rac1, that are important during cell growth and polarity. Human Rit1 shares 95% amino acid identity with mouse and rat Rit1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RIT1
Uniprot