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RIPK2 Antibody

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Western Blot: RIPK2 Antibody [NBP1-86630] - Analysis in human cell line EFO-21.
Western Blot: RIPK2 Antibody [NBP1-86630] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody [NBP1-86630] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86630] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86630] - Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86630] - Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86630] - Immunohistochemical staining of human urinary bladder shows moderate to strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86630] - Immunohistochemical staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RIPK2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RIPK2 Protein (NBP1-86630PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RIPK2 Antibody

  • CARD3
  • CARD-containing IL-1 beta ICE-kinase
  • CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase
  • CARD-containing interleukin-1 beta-converting enzyme-associated kinase
  • CARDIAKCCK
  • EC 2.7.11
  • EC 2.7.11.1
  • GIG30
  • growth-inhibiting gene 30
  • receptor interacting protein 2
  • receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase
  • Receptor-interacting protein 2
  • receptor-interacting serine/threonine-protein kinase 2
  • receptor-interacting serine-threonine kinase 2
  • RICK
  • RICKGIG30
  • RIP2
  • RIP-2
  • RIP2CARD-carrying kinase
  • RIPK2
  • RIP-like-interacting CLARP kinase

Background

RIPK2 encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol RIPK2