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RIPK2 Antibody (1018218) [Unconjugated]

 
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103871) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed ...read more
RIPK2/RIP2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103871) at 5 µg/mL for 1 hour at room temperature followed ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated) using 20 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
1018218
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB103871-SP
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RIPK2 Antibody (1018218) [Unconjugated] Summary

Immunogen
E. coli-derived human RIPK2/RIP2
Met1-Met540
Accession # O43353
Specificity
Detects human RIPK2/RIP2 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK2 Antibody (1018218) [Unconjugated]

  • CARD3
  • CARD-containing IL-1 beta ICE-kinase
  • CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase
  • CARD-containing interleukin-1 beta-converting enzyme-associated kinase
  • CARDIAKCCK
  • EC 2.7.11
  • EC 2.7.11.1
  • GIG30
  • growth-inhibiting gene 30
  • receptor interacting protein 2
  • receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase
  • Receptor-interacting protein 2
  • receptor-interacting serine/threonine-protein kinase 2
  • receptor-interacting serine-threonine kinase 2
  • RICK
  • RICKGIG30
  • RIP2
  • RIP-2
  • RIP2CARD-carrying kinase
  • RIPK2
  • RIP-like-interacting CLARP kinase

Background

Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a  540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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