![Immunocytochemistry/Immunofluorescence: RFX7 Antibody [NBP1-83705] - Staining of human cell line A-431 shows positivity in nucleus and nuclear membrane.](http://images.novusbio.com/fullsize/RFX7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83705-img0003.jpg "Immunocytochemistry/Immunofluorescence: RFX7 Antibody [NBP1-83705] - Staining of human cell line A-431 shows positivity in nucleus and nuclear membrane.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: VVTQHMQSVKQAPKTPQNVPASPGGDRSARHRYPQILPKPANTSALTIRSPTTVLFTSSPIKTAVVPASHMSSLNVVKMTTISLTPSNSNTPLK |
| Predicted Species | Mouse (98%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RFX7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Western_Blot_5226.jpg)
![Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/16351.jpg)
![Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Flow_Cytometry_8189.jpg)
| Publication using NBP1-83705 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RFX7 |
![Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human testis shows moderate nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83705_rabbit-polyclonal-rfx7-antibody-immunohistochemistry-paraffin-310202474156..jpg "Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human testis shows moderate nuclear positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83705_rabbit-polyclonal-rfx7-antibody-immunohistochemistry-paraffin-310202474253..jpg "Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.")
![Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83705_rabbit-polyclonal-rfx7-antibody-immunohistochemistry-paraffin-310202474347..jpg "Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human small intestine shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-83705_rabbit-polyclonal-rfx7-antibody-immunohistochemistry-paraffin-310202474449..jpg "Immunohistochemistry-Paraffin: RFX7 Antibody [NBP1-83705] -Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.")
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Immunocytochemistry/ Immunofluorescence HAPLN1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2608_human-hapln1-affinity-purified-polyclonal-ab-41202412401260.jpg)
![Immunocytochemistry/ Immunofluorescence HAPLN1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2608_human-hapln1-affinity-purified-polyclonal-ab-4120241240223.jpg)
![Immunocytochemistry/ Immunofluorescence HAPLN1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2608_human-hapln1-affinity-purified-polyclonal-ab-4120241239497.jpg)
![Immunohistochemistry CXCL13/BLC/BCA-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CXCL13_AF470_Immunohistochemistry_10193.jpg)
![Neutralization CXCL13/BLC/BCA-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CXCL13_AF470_Block_Neutralize_8888.jpg)
![Immunohistochemistry CXCL13/BLC/BCA-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af470_mouse-cxcl13-blc-bca-1-affinity-purified-polyclonal-ab-8120255561339.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")