![Western Blot: RFX5 Antibody [NBP1-86041] - TEST](http://images.novusbio.com/fullsize/RFX5-Antibody-Western-Blot-NBP1-86041-img0010.jpg "Western Blot: RFX5 Antibody [NBP1-86041] - TEST")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCDWAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLAQPPKDLEART |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RFX5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-86041 | Applications | Species |
|---|---|---|
| Chen DB, Xie XW, Zhao YJ et al. RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A Sci Rep 2020-09-03 [PMID: 32883983] (IF/IHC, Human) | IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for RFX5 Antibody (NBP1-86041)Find related products by research area.
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| Gene Symbol | RFX5 |
![Immunohistochemistry: RFX5 Antibody [NBP1-86041] - The co-expression of RFX5 and KDM4A in HCC tissues was determined by the immunofluorescence staining. The white arrows pointed HCC cells which co-expressed RFX5 and KDM4A. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32883983/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RFX5-Antibody-Immunohistochemistry-NBP1-86041-img0011.jpg "Immunohistochemistry: RFX5 Antibody [NBP1-86041] - The co-expression of RFX5 and KDM4A in HCC tissues was determined by the immunofluorescence staining. The white arrows pointed HCC cells which co-expressed RFX5 and KDM4A. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32883983/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RFX5-Antibody-Immunohistochemistry-Paraffin-NBP1-86041-img0006.jpg "Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RFX5-Antibody-Immunohistochemistry-Paraffin-NBP1-86041-img0007.jpg "Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Kidney shows strong nuclear positivity in cells in tubules and in glomeruli.](http://images.novusbio.com/fullsize/RFX5-Antibody-Immunohistochemistry-Paraffin-NBP1-86041-img0008.jpg "Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Kidney shows strong nuclear positivity in cells in tubules and in glomeruli.")
![Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Lymph node shows strong nuclear positivity in non-germinal center and germinal center cells.](http://images.novusbio.com/fullsize/RFX5-Antibody-Immunohistochemistry-Paraffin-NBP1-86041-img0009.jpg "Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Lymph node shows strong nuclear positivity in non-germinal center and germinal center cells.")
![Immunohistochemistry: RFX5 Antibody [NBP1-86041] - RFX5 is a positive regulator of KDM4A expression in HCC. IHC staining of RFX5 and KDM4A in the same cancer nest. The cellular localization and distribution patterns of KDM4A protein were highly consistent with that of RFX5 protein in the continuous sections of the same cancer nest. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32883983/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-86041_rabbit-polyclonal-rfx5-antibody-20920231721151.jpg "Immunohistochemistry: RFX5 Antibody [NBP1-86041] - RFX5 is a positive regulator of KDM4A expression in HCC. IHC staining of RFX5 and KDM4A in the same cancer nest. The cellular localization and distribution patterns of KDM4A protein were highly consistent with that of RFX5 protein in the continuous sections of the same cancer nest. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32883983/), licensed under a CC-BY license.")
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![N/A CREB [p Ser133] [Biotin]](https://images.novusbio.com/images2/dyc2510-2_human-mouse-rat-phospho-creb-s133-duoset-ic-elisa-2-plate-712021221341.png)
![N/A CREB [p Ser133] [Biotin]](https://images.novusbio.com/images2/dyc2510-2_human-mouse-rat-phospho-creb-s133-duoset-ic-elisa-2-plate-712021221447.png)
![N/A CREB [p Ser133] [Biotin]](https://images.novusbio.com/images2/dyc2510-2_human-mouse-rat-phospho-creb-s133-duoset-ic-elisa-2-plate-712021221551.png)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")