Recombinant Rat Jagged 1 His Tagged Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins (1). Jagged 1 is synthesized as a precursor protein that contains a 33 amino acid (aa) signal sequence, a 1034 aa extracellular region, a 26 aa transmembrane (TM) segment and a 126 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats (2). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%. The extracellular region of Rat Jagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (3). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (4). For instance, soluble non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (5). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (6). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (7). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (8).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.

Showing Publication 1 - 1 of 1.

Publication using 9907-JG	Applications	Species
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Bioassay, Mouse)	Bioassay	Mouse

Array 9907-JG

• Jagged 1 Antibodies
• Jagged 1 ELISA Kit
• Jagged 1 Lysate
• Jagged 1 Protein
• Jagged 1 ELISAs
• Jagged 1 Proteins and Enzymes