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Recombinant Porcine TNF-alpha Protein

 (10 Publications)
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Product Details

Summary
Reactivity PoSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
690-PT-025/CF
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Recombinant Porcine TNF-alpha Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using TNF-susceptible PK‑15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221; Bertoni et al. (1993) J. Immunol. Meth. 160:267. The ED50 for this effect is 0.003-0.018 ng/mL.
Source
E. coli-derived porcine TNF-alpha protein
Arg78-Leu232, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
690-PT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine TNF-alpha Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Porcine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 176 aa extracellular domain (ECD) (3). Within the ECD, porcine TNF-alpha shares 69%-86% aa sequence identity with bovine, canine, cotton rat, equine, feline, human, mouse, rat, and rhesus TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pauli, U. et al. (1989) Gene 81:185.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Recombinant
Porcine TNF-alpha Pro
690-PT
Recombinant Porcine TNF-alpha Protein
Species: Po
Applications: Bioactivity

Publications for TNF-alpha (690-PT)(10)

We have publications tested in 2 confirmed species: Porcine, Transgenic Porcine.

We have publications tested in 4 applications: Activation, Bioassay, ELISA (Standard), ELISA Standard.


Filter By Application
Activation
(1)
Bioassay
(6)
ELISA (Standard)
(2)
ELISA Standard
(1)
All Applications
Filter By Species
Porcine
(8)
Transgenic Porcine
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 690-PT Applications Species
M Liniger, M Gerber, S Renzullo, O García-Nic, N Ruggli TNF-Mediated Inhibition of Classical Swine Fever Virus Replication Is IRF1-, NF-kappaB- and JAK/STAT Signaling-Dependent Viruses, 2021-10-07;13(10):. 2021-10-07 [PMID: 34696447] (Bioassay, Porcine, Transgenic Porcine) Bioassay Porcine, Transgenic Porcine
FS Hohnstein, M Meurer, N de Buhr, M von Köckri, CG Baums, G Alber, N Schütze Analysis of Porcine Pro- and Anti-Inflammatory Cytokine Induction by S. suis In Vivo and In Vitro Pathogens, 2020-01-03;9(1):. 2020-01-03 [PMID: 31947746] (Bioassay, Porcine) Bioassay Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Standard, Porcine) ELISA Standard Porcine
G Franzoni, SP Graham, G Sanna, P Angioi, MS Fiori, A Anfossi, M Amadori, S Dei Giudic, A Oggiano Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence Vet. Microbiol., 2018-02-18;216(0):190-197. 2018-02-18 [PMID: 29519515] (Activation, Porcine) Activation Porcine
H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 2018-03-02;0(0):. 2018-03-02 [PMID: 29502802] (Bioassay, Porcine) Bioassay Porcine
Clyne AM, Zhu H, Edelman ER Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose. J. Cell. Physiol., 2008-10-01;217(1):86-92. 2008-10-01 [PMID: 18446810] (Bioassay, Porcine) Bioassay Porcine
McNulty AL, Moutos FT, Weinberg JB, Guilak F Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha. Arthritis Rheum., 2007-09-01;56(9):3033-42. 2007-09-01 [PMID: 17729298] (Bioassay, Porcine) Bioassay Porcine
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006-05-01;69(5):391-5. 2006-05-01 [PMID: 16604540] (ELISA (Standard)) ELISA (Standard)
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J. Virol., 2006-01-01;80(1):372-82. 2006-01-01 [PMID: 16352562] (ELISA (Standard)) ELISA (Standard)
Seo SH, Hoffmann E, Webster RG The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses. Virus Res., 2004-07-01;103(1):107-13. 2004-07-01 [PMID: 15163498] (Bioassay, Porcine) Bioassay Porcine

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FAQs for TNF-alpha (690-PT). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.
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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
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By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.
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Bioinformatics

Gene Symbol TNF
Uniprot