Home » IL-1 beta/IL-1F2 » IL-1 beta/IL-1F2 Proteins » Recombinant Porcine IL-1 beta/IL-1F2 Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Porcine IL-1 beta/IL-1F2 Protein

 (11 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for IL-1 beta/IL-1F2 (681-PI).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity PoSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
681-PI-010/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Porcine IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.75-4.5 ng/mL.
Source
E. coli-derived porcine IL-1 beta/IL-1F2 protein
Ala115-Pro267
Accession #
N-terminal Sequence
Ala115
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
681-PI in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Huether, M. et al. (1993) Gene 129:285.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(664 Publications)
Species: Mu
Applications: ELISA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA

IL-1ra/IL-1F3/IL1RN [H
DRA00B
N/A IL-1ra/IL-1F3/IL1RN [HRP]
N/A IL-1ra/IL-1F3/IL1RN [HRP]
N/A IL-1ra/IL-1F3/IL1RN [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
IL-1 alpha/IL-1F1
200-LA
SDS-PAGE IL-1 alpha/IL-1F1
Bioactivity IL-1 alpha/IL-1F1
(65 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(454 Publications)
Species: Hu
Applications: BA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(183 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
iNOS Antibody -
BSA Free
NB300-605
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
(3 Reviews)
(130 Publications)
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(217 Publications)
Species: Hu
Applications: BA
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
DBT Antibody
NBP1-89522
Immunocytochemistry/ Immunofluorescence DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(256 Publications)
Species: Hu
Applications: ELISA
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(88 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
Recombinant
Porcine IL-1 beta/IL- ...
681-PI
Recombinant Porcine IL-1 beta/IL-1F2 Protein
Species: Po
Applications: Bioactivity

Publications for IL-1 beta/IL-1F2 (681-PI)(11)

We have publications tested in 2 confirmed species: Bovine, Porcine.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), ELISA Developmet, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(6)
ELISA (Standard)
(1)
ELISA Developmet
(2)
ELISA Standard
(2)
In Vivo
(1)
All Applications
Filter By Species
Bovine
(1)
Porcine
(9)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 681-PI Applications Species
M Dresen, J Schenk, Y Berhanu We, D Vötsch, W Baumgärtne, P Valentin-W, A Nerlich Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue Microorganisms, 2021-02-12;9(2):. 2021-02-12 [PMID: 33673302] (Bioassay, Porcine) Bioassay Porcine
H Cho, FU Bhatti, KA Hasty, AK Yi Nanosome-Mediated Delivery Of Protein Kinase D Inhibitor Protects Chondrocytes From Interleukin-1beta-Induced Stress And Apoptotic Death Int J Nanomedicine, 2019-11-11;14(0):8835-8846. 2019-11-11 [PMID: 31806974] (Bioassay, Porcine) Bioassay Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Standard, Porcine) ELISA Standard Porcine
H Tabeian, BF Betti, C Dos Santos, TJ de Vries, F Lobbezoo, AV Ter Linde, B Zandieh-Do, MI Koenders, V Everts, AD Bakker IL-1? Damages Fibrocartilage and Upregulates MMP-13 Expression in Fibrochondrocytes in the Condyle of the Temporomandibular Joint Int J Mol Sci, 2019-05-07;20(9):. 2019-05-07 [PMID: 31067826] (Bioassay, Porcine) Bioassay Porcine
Park H, Liu G, Liu Q, Zhou Y Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses, 2018-08-09;10(8):. 2018-08-09 [PMID: 30096906] (ELISA Developmet, Porcine) ELISA Developmet Porcine
HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination J. Virol., 2018-03-28;0(0):. 2018-03-28 [PMID: 29386291] (ELISA Standard, ELISA Developmet, Porcine) ELISA Standard, ELISA Developmet Porcine
Comblain F, Sanchez C, Lesponne I, Balligand M, Serisier S, Henrotin Y Curcuminoids extract, hydrolyzed collagen and green tea extract synergically inhibit inflammatory and catabolic mediator&#039;s synthesis by normal bovine and osteoarthritic human chondrocytes in monolayer. PLoS ONE, 2015-03-23;10(3):e0121654. 2015-03-23 [PMID: 25799427] (Bioassay, Bovine) Bioassay Bovine
Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin. J. Interferon Cytokine Res., 2007-09-01;27(9):757-66. 2007-09-01 [PMID: 17892397] (Bioassay, Porcine) Bioassay Porcine
Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2634-43. 2007-06-01 [PMID: 17525194] (Bioassay, Porcine) Bioassay Porcine
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006-05-01;69(5):391-5. 2006-05-01 [PMID: 16604540] (ELISA (Standard)) ELISA (Standard)
Show All 11 Publications.

Reviews for IL-1 beta/IL-1F2 (681-PI) (0)

There are no reviews for IL-1 beta/IL-1F2 (681-PI). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-1 beta/IL-1F2 (681-PI) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-1 beta/IL-1F2 Products

Blogs on IL-1 beta/IL-1F2.

Check out the latest blog posts on IL-1 beta/IL-1F2.
The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.
Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL-1 beta/IL-1F2 Antibody [Unconjugated]
AF-401-NA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Porcine IL-1 beta/IL-1F2 Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol IL1B1
Uniprot