| Reactivity | MuSpecies Glossary |
| Applications | Bioactivity |
| Details of Functionality | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL. |
| Source | Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-8a protein Ala20-Trp354 |
| Accession # | |
| N-terminal Sequence | Ala20 & Trp23 |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | Wnt8a |
| Purity | >95%, by SDS-PAGE with silver staining. |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| SDS-PAGE | 40-62 kDa, reducing conditions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein. |
| Purity | >95%, by SDS-PAGE with silver staining. |
| Reconstitution Instructions | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Wnt-8a is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-8a consists of a 19 amino acid (aa) signal peptide that is cleaved to release the mature 335 aa secreted protein (2). Mature mouse Wnt-8a shares 82% and 91% sequence identity with human and rat mature Wnt-8a, respectively. Similar to other canonical Wnts, Wnt-8a binds to the Frizzled family of receptors to intiate beta-catenin signaling. Wnt-8a is expressed during early embryogenesis and is involved in mesoderm patterning and posteriorization of the neuroectoderm (3-6). Wnt-8a signaling promotes optic lens development as well as otic placode formation during inner ear development (7, 8). Expression of Wnt-8a is observed in heart progenitor cells where it positively regulates cardiac myogenesis (9, 10).
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