Recombinant Mouse VEGFR1/Flt-1 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs) (1, 2). While family members VEGFR1, VEGFR2/KDR/Flk-1 and VEGFR3/Flt-4 are all mainly expressed on endothelial cells and play central roles in vasculogenesis, angiogenesis, and lymphangiogenesis, only VEGFR1 is expressed on macrophages, and mainly plays inhibitory roles (1-3). VEGFR1 expression is also reported on osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells (1, 2). Like other class III RTKs, mouse VEGFR1 contains a signal peptide (aa 1-22), an extracellular domain (ECD aa 23-759) with seven Ig-like repeats, a transmembrane domain (aa 760-781) and a cytoplasmic region (aa 782-1333) with a tyrosine kinase domain and several autocatalytic phosphotyrosine sites. Mouse VEGFR1 ECD shares 91% aa sequence identity with rat and 76-79% with human, equine, canine and porcine VEGFR1. Soluble forms of the VEGFR1 ECD are produced by alternative splicing, and may also be shed during regulated intracellular proteolysis (4-10). Both soluble and transmembrane forms can inhibit angiogenesis by binding and sequestering its ligands, VEGF (VEGF-A), VEGF-B or PlGF (6-11). VEGFR1 dimerizes upon ligand binding, which can include heterodimerization with VEGFR2 that modifies VEGFR2-mediated endothelial proliferation and vessel branching (8, 11, 12). VEGFR1 binds VEGF with higher affinity than does VEGFR2, but shows weaker kinase activity (9, 13). Both PlGF and VEGF induce autophosphorylation of transmembrane VEGFR1 (5, 9, 13). While deletion of mouse VEGFR1 is lethal due to overgrowth and disorganization of the vasculature, kinase-inactive mutants are viable (13, 14). VEGFR1 is up‑regulated during hypoxia, and participates in neovascularization and wound healing (1, 2, 15). VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines (1, 3, 13, 16). Lymphangiogenesis, angiogenesis, and growth-promoting effects of VEGFR1 are thought to result from enhanced migration of macrophages from the bone marrow to tumors and tissues where they recruit endothelial progenitors (3, 16). Circulating levels of VEGFR1 increase during pregnancy and are further elevated in preeclampsia (4, 6, 17).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), Functional Assay, In Vivo, Surface Plasmon Resonance.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using 471-F1	Applications	Species
X Huang, L Jia, Y Jia, X Xu, R Wang, M Wei, H Li, H Peng, Y Wei, Q He, K Wang sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in mice Cytokine, 2023-04-14;166(0):156190. 2023-04-14 [PMID: 37062152] (Bioassay, Mouse)	Bioassay	Mouse
N Popovic, E Hooker, A Barabino, A Flamier, F Provost, M Buscarlet, G Bernier, B Larrivée COCO/DAND5 inhibits developmental and pathological ocular angiogenesis Embo Molecular Medicine, 2021-02-15;0(0):e12005. 2021-02-15 [PMID: 33587337] (Bioassay, Mouse)	Bioassay	Mouse
B Di Marco, EE Crouch, B Shah, C Duman, MF Paredes, C Ruiz de Al, EJ Huang, J Alfonso Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon Cell Rep, 2020-10-13;33(2):108256. 2020-10-13 [PMID: 33053356] (Bioassay, Mouse)	Bioassay	Mouse
V Mashayekhi, KT Xenaki, PMP van Bergen, S Oliveira Dual Targeting of Endothelial and Cancer Cells Potentiates In Vitro Nanobody-Targeted Photodynamic Therapy Cancers, 2020-09-23;12(10):. 2020-09-23 [PMID: 32977602] (Functional Assay, Mouse)	Functional Assay	Mouse
M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy PLoS Genet., 2019-12-26;15(12):e1008468. 2019-12-26 [PMID: 31877123] (Surface Plasmon Resonance, Mouse)	Surface Plasmon Resonance	Mouse
G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019-05-28;10(1):2350. 2019-05-28 [PMID: 31138815] (Cell Culture, Mouse)	Cell Culture	Mouse
DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor PLoS ONE, 2018-12-19;13(12):e0208579. 2018-12-19 [PMID: 30566445] (In Vivo, Mouse)	In Vivo	Mouse
T Ikeuchi, S de Vega, P Forcinito, AD Doyle, J Amaral, IR Rodriguez, E Arikawa-Hi, Y Yamada Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity Sci Rep, 2018-12-05;8(1):17654. 2018-12-05 [PMID: 30518776] (Bioassay, Human)	Bioassay	Human
S Atienzar-A, G Serrano-He, A Freire Val, C Ruiz de Al, M Muriach, JM Barcia, JM Garcia-Ver, FJ Romero, J Sancho-Pel Role of retinal pigment epithelium-derived exosomes and autophagy in new blood vessel formation J. Cell. Mol. Med., 2018-08-21;0(0):. 2018-08-21 [PMID: 30133118] (Bioassay, Human)	Bioassay	Human
JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease Exp. Hematol., 2018-01-11;0(0):. 2018-01-11 [PMID: 29337222] (Binding Assay, Mouse)	Binding Assay	Mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015-01-28;10(1):e0117908. 2015-01-28 [PMID: 25629162] (Bioassay, Mouse)	Bioassay	Mouse
Liu , Hua, Zhang , Wenbo, Xu , Zhimin, Caldwell , Robert W, Caldwell , Ruth B, Brooks , Steven E Hyperoxia causes regression of vitreous neovascularization by downregulating VEGF/VEGFR2 pathway. Invest Ophthalmol Vis Sci, 2013-02-01;54(2):918-31. 2013-02-01 [PMID: 23307955] (In Vivo, Mouse)	In Vivo	Mouse
Abramovich D, Irusta G, Bas D, Cataldi NI, Parborell F, Tesone M Angiopoietins/TIE2 system and VEGF are involved in ovarian function in a DHEA rat model of polycystic ovary syndrome. Endocrinology, 2012-05-10;153(7):3446-56. 2012-05-10 [PMID: 22577112] (In Vivo, Rat)	In Vivo	Rat
Fukushima Y, Okada M, Kataoka H Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice. J. Clin. Invest., 2011-04-18;121(5):1974-85. 2011-04-18 [PMID: 21505259] (In Vivo, Mouse)	In Vivo	Mouse
Tam Tam KB, Lamarca B, Arany M, Cockrell K, Fournier L, Murphy S, Martin JN, Granger JP Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats. Am. J. Hypertens., 2010-08-19;24(1):110-3. 2010-08-19 [PMID: 20725052] (In Vivo, Rat)	In Vivo	Rat
Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1. J. Biol. Chem., 2008-10-15;283(49):34250-9. 2008-10-15 [PMID: 18922791] (Binding Assay, Mouse)	Binding Assay	Mouse
Kubota Y, Hirashima M, Kishi K, Stewart CL, Suda T Leukemia inhibitory factor regulates microvessel density by modulating oxygen-dependent VEGF expression in mice. J. Clin. Invest., 2008-07-01;118(7):2393-403. 2008-07-01 [PMID: 18521186] (In Vivo, Mouse)	In Vivo	Mouse
Tei K, Matsumoto T, Mifune Y, Ishida K, Sasaki K, Shoji T, Kubo S, Kawamoto A, Asahara T, Kurosaka M, Kuroda R Administrations of peripheral blood CD34-positive cells contribute to medial collateral ligament healing via vasculogenesis. Stem Cells, 2008-01-10;26(3):819-30. 2008-01-10 [PMID: 18192236] (In Vivo, Mouse)	In Vivo	Mouse
Tsao PN, Chan FT, Wei SC, Hsieh WS, Chou HC, Su YN, Chen CY, Hsu WM, Hsieh FJ, Hsu SM Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis. Crit. Care Med., 2007-08-01;35(8):1955-60. 2007-08-01 [PMID: 17568329] (In Vivo, Mouse)	In Vivo	Mouse
Ambati BK, Nozaki M, Singh N, Takeda A, Jani PD, Suthar T, Albuquerque RJ, Richter E, Sakurai E, Newcomb MT, Kleinman ME, Caldwell RB, Lin Q, Ogura Y, Orecchia A, Samuelson DA, Agnew DW, St Leger J, Green WR, Mahasreshti PJ, Curiel DT, Kwan D, Marsh H, Ikeda S, Leiper LJ, Collinson JM, Bogdanovich S, Khurana TS, Shibuya M, Baldwin ME, Ferrara N, Gerber HP, De Falco S, Witta J, Baffi JZ, Raisler BJ, Ambati J Corneal avascularity is due to soluble VEGF receptor-1. Nature, 2006-10-18;443(7114):993-7. 2006-10-18 [PMID: 17051153] (In Vivo, Mouse)	In Vivo	Mouse
Matsumoto T, Kawamoto A, Kuroda R, Ishikawa M, Mifune Y, Iwasaki H, Miwa M, Horii M, Hayashi S, Oyamada A, Nishimura H, Murasawa S, Doita M, Kurosaka M, Asahara T Therapeutic potential of vasculogenesis and osteogenesis promoted by peripheral blood CD34-positive cells for functional bone healing. Am. J. Pathol., 2006-10-01;169(4):1440-57. 2006-10-01 [PMID: 17003498] (In Vivo, Rat)	In Vivo	Rat
Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 2006-10-01;169(4):1167-82. 2006-10-01 [PMID: 17003476] (ELISA (Standard))	ELISA (Standard)
Niida S, Kondo T, Hiratsuka S, Hayashi S, Amizuka N, Noda T, Ikeda K, Shibuya M VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc. Natl. Acad. Sci. U.S.A., 2005-09-19;102(39):14016-21. 2005-09-19 [PMID: 16172397] (In Vivo, Mouse)	In Vivo	Mouse
Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue. J. Lab. Clin. Med., 2005-04-01;145(4):194-203. 2005-04-01 [PMID: 15962838] (In Vivo, Rat)	In Vivo	Rat
Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J. Cell Biol., 2003-06-16;161(6):1163-77. 2003-06-16 [PMID: 12810700] (In Vivo, Rat)	In Vivo	Rat
Sugimoto H, Hamano Y, Charytan D, Cosgrove D, Kieran M, Sudhakar A, Kalluri R Neutralization of circulating vascular endothelial growth factor (VEGF) by anti-VEGF antibodies and soluble VEGF receptor 1 (sFlt-1) induces proteinuria. J. Biol. Chem., 2003-01-21;278(15):12605-8. 2003-01-21 [PMID: 12538598] (In Vivo, Mouse)	In Vivo	Mouse
Show All 26 Publications. Collapse Publications.

Array 471-F1

• VEGFR1/Flt-1 Antibodies
• VEGFR1/Flt-1 ELISA Kits
• VEGFR1/Flt-1 Proteins
• VEGFR1/Flt-1 Proteins and Enzymes
• VEGFR1/Flt-1 ELISAs