| Reactivity | MuSpecies Glossary |
| Applications | Bioactivity |
| Details of Functionality | Measured by its ability to inhibit LPS-induced TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. 3.3 μg/mL of rmPLTP will inhibit 65-95% of the TNF-alpha secretion induced by 0.5 ng/mL of LPS. |
| Source | Mouse myeloma cell line, NS0-derived mouse PLTP protein Glu18-Ala493, with a C-terminal 6-His tag |
| Accession # | |
| N-terminal Sequence | Glu18 |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | Pltp |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 53.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| SDS-PAGE | 70-85 kDa, reducing conditions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions | Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Phospholipid transfer protein (PLTP; also known as lipid transfer protein II) is an 80 kDa, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily of molecules (1). It is synthesized as a precursor with a 17 amino acid (aa) signal sequence and a 476 aa mature chain. The mature chain contains two BPI/LBP/CETP domains (aa 21 - 239 and 263 - 463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4 - 6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha -tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7 - 11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).
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![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](https://images.novusbio.com/images/PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg "Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.")