Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Osteoprotegerin (OPG), also called OCIF (osteoclastogenesis inhibitory factor) is a secreted 55-60 kDa protein that regulates bone density (1-3). As a member of the tumor necrosis factor receptor (TNFR) superfamily of proteins, it is designated TNFRSF11B (1-4). Mouse OPG cDNA encodes 401 amino acids (aa) including a 21 aa signal peptide and a 380 aa mature soluble protein with four TNFR domains, two death domains and a heparin‑binding region (4). The cysteine‑rich TNFR domains are essential for ligand interaction, while a cysteine at the C‑terminus mediates homodimerization (4). Mature mouse OPG shares 86%, 94%, 86%, 86% and 83% amino acid sequence identity with human, rat, equine, canine and bovine OPG, respectively. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts and endothelial cells (1, 2, 5, 7). Regulation of its expression by estrogen, parathyroid hormone and cytokines is complex and changes with age (2). OPG has been called a decoy receptor for the TNF superfamily ligands, TRANCE (tumor necrosis factor‑related activation‑induced cytokine), also called RANK L (receptor activator of NF kappa B ligand), and TRAIL (TNF‑related apoptosis‑inducing ligand), which also bind TNF family receptors RANK and TRAIL receptors 1-4, respectively (2, 6). TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL‑induced apoptosis (5, 6). Expression of RANK L on the cell surface, and thus its ability to stimulate osteoclastogenesis, is regulated by OPG by intracellular and extracellular mechanisms (7). Within osteoblasts, interaction of the basic domain of OPG with RANK L in the Golgi inhibits RANK L secretion (7). Extracellularly, OPG binding to RANK L results in clathrin‑mediated internalization and degradation of both proteins (7, 8). Binding of OPG by syndecan‑1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss (8, 9). OPG deficiency can cause juvenile Paget’s disease in humans, and insufficient OPG to balance with RANK L and RANK can produce osteoporosis and vascular calcification in both mice and humans (2, 10, 11).

We have publications tested in 4 confirmed species: Mouse, Rat, Avian - Quail, Duck.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Neutralization, Tissue Culture, Western Blot.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using 459-MO	Applications	Species
Liao, M;Zhu, X;Lu, Y;Yi, X;Hu, Y;Zhao, Y;Ye, Z;Guo, X;Liang, M;Jin, X;Zhang, H;Wang, X;Zhao, Z;Chen, Y;Yan, H; Multi-omics profiling of retinal pigment epithelium reveals enhancer-driven activation of RANK-NFATc1 signaling in traumatic proliferative vitreoretinopathy Nature communications 2024-08-25 [PMID: 39183203] (Bioassay, Mouse)	Bioassay	Mouse
Park, SH;Peters, M;Aguayo, C;Farris, MK;Hughes, RT;Moore, J;Munley, MT;Reno, KE;Gardin, J;Cline, JM;Peters, CM;Willey, JS; Secretory factors released from high dose radiation-activated osteoclasts increase the expression level of pain-associated neuropeptides in sensory neuronal cultures Research square 2024-07-01 [PMID: 39011106] (Bioassay, Mouse)	Bioassay	Mouse
J Zhu, Y Ma, J Wang, Y Wang, W Ali, H Zou, H Zhao, X Tong, R Song, Z Liu The Mechanism of Osteoprotegerin-Induced Osteoclast Pyroptosis In Vitro International Journal of Molecular Sciences, 2023-01-12;24(2):. 2023-01-12 [PMID: 36675029] (Cell Culture, Mouse)	Cell Culture	Mouse
JM Kim, YS Yang, J Xie, O Lee, J Kim, J Hong, B Boldyreff, O Filhol, H Chun, MB Greenblatt, G Gao, JH Shim Regulation of sclerostin by the SIRT1 stabilization pathway in osteocytes Cell Death and Differentiation, 2022-02-15;0(0):. 2022-02-15 [PMID: 35169297] (Bioassay, Mouse)	Bioassay	Mouse
MS AlQranei, LT Senbanjo, H Aljohani, T Hamza, MA Chellaiah Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling BMC immunology, 2021-03-25;22(1):23. 2021-03-25 [PMID: 33765924] (Bioassay, Mouse)	Bioassay	Mouse
M Paolino, R Koglgruber, SJF Cronin, I Uribesalgo, E Rauscher, J Harreiter, M Schuster, D Bancher-To, B Pranjic, M Novatchkov, JP Fededa, AJ White, V Sigl, S Dekan, T Penz, C Bock, L Kenner, GA Holländer, G Anderson, A Kautzky-Wi, JM Penninger RANK links thymic regulatory T cells to fetal loss and gestational diabetes in pregnancy Nature, 2020-12-23;0(0):. 2020-12-23 [PMID: 33361811] (Cell Culture, Mouse)	Cell Culture	Mouse
X Tong, M Chen, R Song, H Zhao, J Bian, J Gu, Z Liu Overexpression of c-Fos reverses osteoprotegerin-mediated suppression of osteoclastogenesis by increasing the Beclin1-induced autophagy Journal of Cellular and Molecular Medicine, 2020-12-04;0(0):. 2020-12-04 [PMID: 33277741] (Cell Culture, Mouse)	Cell Culture	Mouse
A Adhyatmika, L Beljaars, KSS Putri, H Habibie, CE Boorsma, C Reker-Smit, T Luangmonko, B Guney, A Haak, KA Mangnus, E Post, K Poelstra, K Ravnskjaer, P Olinga, BN Melgert Osteoprotegerin is More than a Possible Serum Marker in Liver Fibrosis: A Study into its Function in Human and Murine Liver Pharmaceutics, 2020-05-21;12(5):. 2020-05-21 [PMID: 32455750] (Tissue Culture, Mouse)	Tissue Culture	Mouse
T Hasegawa, J Kikuta, T Sudo, Y Matsuura, T Matsui, S Simmons, K Ebina, M Hirao, D Okuzaki, Y Yoshida, A Hirao, VV Kalinichen, K Yamaoka, T Takeuchi, M Ishii Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1 Nat. Immunol., 2019-11-18;20(12):1631-1643. 2019-11-18 [PMID: 31740799] (Cell Culture, Mouse)	Cell Culture	Mouse
X Gao, J Zheng, S Tu, B Cai, R Zeng, L Xiang Role of osteoprotegerin in the regulation of dental epithelial?mesenchymal signaling during tooth development Mol Med Rep, 2019-08-07;20(4):3035-3042. 2019-08-07 [PMID: 31432164] (Bioassay, Mouse)	Bioassay	Mouse
SS Dufresne, A Boulanger-, S Bossé, A Argaw, D Hamoudi, L Marcadet, D Gamu, VA Fajardo, H Yagita, JM Penninger, A Russell Tu, J Frenette Genetic deletion of muscle RANK or selective inhibition of RANKL is not as effective as full-length OPG-fc in mitigating muscular dystrophy Acta Neuropathol Commun, 2018-04-24;6(1):31. 2018-04-24 [PMID: 29699580] (In Vivo, Mouse)	In Vivo	Mouse
JJ Barrott, BE Illum, H Jin, ML Hedberg, Y Wang, A Grossmann, M Haldar, MR Capecchi, KB Jones Paracrine osteoprotegerin and ?-catenin stabilization support synovial sarcomagenesis in periosteal cells J. Clin. Invest., 2017-11-20;0(0):. 2017-11-20 [PMID: 29202462] (In Vivo, Mouse)	In Vivo	Mouse
S Bernardi, B Toffoli, F Bossi, R Candido, E Stenner, R Carretta, F Barbone, B Fabris Circulating osteoprotegerin is associated with chronic kidney disease in hypertensive patients BMC Nephrol, 2017-07-06;18(1):219. 2017-07-06 [PMID: 28683789] (In Vivo, Mouse)	In Vivo	Mouse
Ealba E, Jheon A, Hall J, Curantz C, Butcher K, Schneider R Neural crest-mediated bone resorption is a determinant of species-specific jaw length. Dev Biol, 2015-10-21;408(1):151-63. 2015-10-21 [PMID: 26449912] (In Vivo, Avian - Quail, Duck)	In Vivo	Avian - Quail, Duck
Matsushita M, Ozaki Y, Hasegawa Y, Terada F, Tabata N, Shiheido H, Yanagawa H, Oikawa T, Matsuo K, Du W, Yamada T, Hozumi M, Ichikawa D, Hattori Y A novel phthalimide derivative, TC11, has preclinical effects on high-risk myeloma cells and osteoclasts. PLoS ONE, 2015-01-24;10(1):e0116135. 2015-01-24 [PMID: 25617756] (Bioassay, Mouse)	Bioassay	Mouse
Mukai T, Ishida S, Ishikawa R, Yoshitaka T, Kittaka M, Gallant R, Lin Y, Rottapel R, Brotto M, Reichenberger E, Ueki Y SH3BP2 cherubism mutation potentiates TNF-alpha-induced osteoclastogenesis via NFATc1 and TNF-alpha-mediated inflammatory bone loss. J Bone Miner Res, 2014-12-01;29(12):2618-35. 2014-12-01 [PMID: 24916406] (Neutralization, Mouse)	Neutralization	Mouse
Takeshita S, Fumoto T, Naoe Y, Ikeda K Age-related marrow adipogenesis is linked to increased expression of RANKL. J Biol Chem, 2014-04-21;289(24):16699-710. 2014-04-21 [PMID: 24753250] (Bioassay, Mouse)	Bioassay	Mouse
Rementer C, Wu M, Buranaphatthana W, Yang H, Scatena M, Giachelli C An inducible, ligand-independent receptor activator of NF-kappaB gene to control osteoclast differentiation from monocytic precursors. PLoS ONE, 2013-12-27;8(12):e84465. 2013-12-27 [PMID: 24386387] (Bioassay, Mouse)	Bioassay	Mouse
Charles , Julia F, Hsu , Lih-Yun, Niemi , Erene C, Weiss , Arthur, Aliprantis , Antonios, Nakamura , Mary C Inflammatory arthritis increases mouse osteoclast precursors with myeloid suppressor function. J Clin Invest, 2012-11-01;122(12):4592-605. 2012-11-01 [PMID: 23114597] (Bioassay, Mouse)	Bioassay	Mouse
Onal M, Xiong J, Chen X, Thostenson J, Almeida M, Manolagas S Receptor activator of nuclear factor kappaB ligand (RANKL) protein expression by B lymphocytes contributes to ovariectomy-induced bone loss. Oncogene, 2012-07-10;287(35):29851-60. 2012-07-10 [PMID: 22782898] (Bioassay, Mouse)	Bioassay	Mouse
Schrader J, Rennekamp W, Niebergall U, Schoppet M, Jahr H, Brendel MD, Horsch D, Hofbauer LC Cytokine-induced osteoprotegerin expression protects pancreatic beta cells through p38 mitogen-activated protein kinase signalling against cell death. Diabetologia, 2007-04-19;50(6):1243-7. 2007-04-19 [PMID: 17443309] (Bioassay, Rat)	Bioassay	Rat
Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts. J. Immunol., 2007-01-01;178(1):192-200. 2007-01-01 [PMID: 17182555] (Bioassay, Mouse)	Bioassay	Mouse
Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK JunB as a downstream mediator of PTHrP actions in cementoblasts. J. Bone Miner. Res., 2005-11-07;21(2):246-57. 2005-11-07 [PMID: 16418780] (Bioassay, Mouse)	Bioassay	Mouse
Gustafsson BI, Thommesen L, Stunes AK, Tommeras K, Westbroek I, Waldum HL, Slordahl K, Tamburstuen MV, Reseland JE, Syversen U Serotonin and fluoxetine modulate bone cell function in vitro. J. Cell. Biochem., 2006-05-01;98(1):139-51. 2006-05-01 [PMID: 16408289] (ELISA (Standard))	ELISA (Standard)
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA (Standard))	ELISA (Standard)
Cao J, Venton L, Sakata T, Halloran BP Expression of RANKL and OPG correlates with age-related bone loss in male C57BL/6 mice. J. Bone Miner. Res., 2003-02-01;18(2):270-7. 2003-02-01 [PMID: 12568404] (Western Blot)	Western Blot
Show All 26 Publications. Collapse Publications.

Array 459-MO

• Osteoprotegerin/TNFRSF11B Antibodies
• Osteoprotegerin/TNFRSF11B ELISA Kits
• Osteoprotegerin/TNFRSF11B Proteins
• Osteoprotegerin/TNFRSF11B Proteins and Enzymes
• Osteoprotegerin/TNFRSF11B ELISAs