Home » Natriuretic Peptide Receptor C » Natriuretic Peptide Receptor C Proteins » Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6‑3.6 ng/mL.
When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6‑3.6 ng/mL.
2 μg/lane of Recombinant Mouse INPRC/NPR3 Fc Chimera was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® blue staining, showing bands at 76-96 kDa and 170-200 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals offers 100% Guarantee

Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin (Catalog # 9700-ON) that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse NPRC/NPR3 protein
Mouse NPRC/NPR3
(Glu41-Ala478)
Accession # P70180		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence

Glu41
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
76-96 kDa, under reducing conditions
Publications
Read Publication using
10187-NR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

  • ANP-C
  • ANPR-C
  • ANPRCFLJ14054
  • Atrial natriuretic peptide clearance receptor
  • atrial natriuretic peptide receptor 3
  • Atrial natriuretic peptide receptor type C
  • C5orf23
  • chromosome 5 open reading frame 23
  • GUCY2B
  • lgj
  • natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C)
  • NPR3
  • NPRC
  • NPR-C
  • stri

Background

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPR-C or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mouse NPR3 is a type I transmembrane glycoprotein that contains a 438 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 37 aa cytoplasmic region. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).
  1. Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
  2. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
  3. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
  4. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.

Customers Who Viewed This Item Also Viewed...

preproANP Antibody
NBP2-14873
Immunohistochemistry-Paraffin preproANP Antibody
Immunohistochemistry-Paraffin preproANP Antibody
Western Blot preproANP Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NPRA/NPR1 Antibody
NBP1-31333
Western Blot NPRA/NPR1 Antibody
Immunohistochemistry-Paraffin NPRA/NPR1 Antibody
Immunohistochemistry NPRA/NPR1 Antibody
(3 Publications)
Species: Hu, Mu, Rb, Rt
Applications: IHC, IHC-P, WB
NPRB/NPR2 Antibody
NBP3-12239
Immunohistochemistry-Paraffin NPRB/NPR2 Antibody
Western Blot NPRB/NPR2 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
CNPase Antibody
(CL2887)
NBP2-46617
Immunohistochemistry CNPase Antibody (CL2887)
Immunohistochemistry CNPase Antibody (CL2887)
Immunohistochemistry-Paraffin CNPase Antibody (CL2887)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NT-proBNP Antibody
(OTI11G4)
NBP2-46201
Western Blot NT-proBNP Antibody (OTI11G4)
Immunohistochemistry NT-proBNP Antibody (OTI11G4)
Immunohistochemistry NT-proBNP Antibody (OTI11G4)
(1 Review)
Species: Hu
Applications: IHC, IHC-P, WB
Neuronal Pentraxin
R/NPTXR Antibody [Unc ...
AF4414
Simple Western Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]
Immunohistochemistry Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]
(6 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Neprilysin/CD10
Antibody [Unconjugate
AF1126
Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
(22 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
iNOS Antibody -
BSA Free
NB300-605
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
(3 Reviews)
(136 Publications)
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
HESX1 Antibody
NBP1-89090
Immunohistochemistry-Paraffin HESX1 Antibody
Immunohistochemistry-Paraffin HESX1 Antibody
Immunohistochemistry-Paraffin HESX1 Antibody
Species: Hu
Applications: IHC, IHC-P
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Desmin Antibody
[Unconjugated]
AF3844
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: IHC
eNOS Antibody -
BSA Free
NB300-500
Western Blot eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
(3 Reviews)
(29 Publications)
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
TUSC4 Antibody
NBP1-82537
Western Blot TUSC4 Antibody
Immunocytochemistry/ Immunofluorescence TUSC4 Antibody
Immunohistochemistry-Paraffin TUSC4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Recombinant Mouse
NPRC/NPR3 Fc Chimera ...
10187-NR
Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF
Species: Mu
Applications: Bioactivity

Publications for Natriuretic Peptide Receptor C (10187-NR)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Natriuretic Peptide Receptor C (10187-NR) (0)

There are no reviews for Natriuretic Peptide Receptor C (10187-NR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Natriuretic Peptide Receptor C (10187-NR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Natriuretic Peptide Receptor C Products

Blogs on Natriuretic Peptide Receptor C

There are no specific blogs for Natriuretic Peptide Receptor C, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
iNOS Antibody - BSA Free
NB300-605
Western Blot: eNOS Antibody - BSA Free [NB300-500] - Fractionated, compared to single exposure, radiation more profoundly suppressed TM and eNOS. Representative Western blot analysis and quantification of KLF2 (n = 5) and KLF4 (n = 3) levels in whole-cell lysates from nonirradiated (sham) and irradiated HUVECs 4 h and 24 h after exposure to either five fractions of 2 Gy (5 x 2 Gy) or single exposure to 10 Gy. Fractions delivered at 24-h intervals. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091/) licensed under a CC-BY license.
eNOS Antibody - BSA Free
NB300-500
Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10&nbsp;µg/mL overnight at 4&nbsp;°C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Desmin Antibody [Unconjugated]
AF3844

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF and receive a gift card or discount.


Bioinformatics

Uniprot