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Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

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When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6‑3.6 ng/mL.
When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6‑3.6 ng/mL.
2 μg/lane of Recombinant Mouse INPRC/NPR3 Fc Chimera was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® blue staining, showing bands at 76-96 kDa and 170-200 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin (Catalog # 9700-ON) that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse NPRC/NPR3 protein
Mouse NPRC/NPR3
(Glu41-Ala478)
Accession # P70180		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence

Glu41
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
76-96 kDa, under reducing conditions
Publications
Read Publication using
10187-NR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

  • ANP-C
  • ANPR-C
  • ANPRCFLJ14054
  • Atrial natriuretic peptide clearance receptor
  • atrial natriuretic peptide receptor 3
  • Atrial natriuretic peptide receptor type C
  • C5orf23
  • chromosome 5 open reading frame 23
  • GUCY2B
  • lgj
  • natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C)
  • NPR3
  • NPRC
  • NPR-C
  • stri

Background

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPR-C or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mouse NPR3 is a type I transmembrane glycoprotein that contains a 438 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 37 aa cytoplasmic region. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).
  1. Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
  2. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
  3. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
  4. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.

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Recombinant Mouse
NPRC/NPR3 Fc Chimera ...
10187-NR
Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF
Species: Mu
Applications: Bioactivity

Publications for Natriuretic Peptide Receptor C (10187-NR)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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