Name: Recombinant Mouse Complement Component C5a Protein, CF
Brand: Novus Biologicals
SKU: 2150-C5/CF
Price: 400 USD

- COVID-19 Research Tools
  
  Antibodies
  Primary Antibodies
  Conjugated Antibodies
  
  Sample Size Antibodies
  
  KO Validated Antibodies
  
  Recombinant Antibodies
  
  Antibody Sampler Packs
  
  Antibody Pairs
  
  Secondary Antibodies
  
  Isotype Control Antibodies
  
  Proteins and Peptides
  Peptides & Proteins
  
  Blocking Peptides
  
  Biologically Active Proteins
  
  Lysates and Cell Lines
  Lysates
  
  Stem Cell Lines
- Kits
  Antibody Labeling Kits
  
  ChIP Kits
  
  ELISA Kits
  
  Flow Cytometry Kits
  
  Activators and Inhibitors
  Inhibitors
  
  Activators
  
  Support Products
  Exosome Isolation
  
  Flow Cytometry Beads
  
  IHC Tissue Slides
  
  IHC Microarrays
  
  Western Blot Gels & Buffers
  
  Western Blot Membranes
  
  All Support Products
  
  Bulk and Custom
Products
Our Protocols
- Resources
  Protocols
  
  Video Protocols
  
  Troubleshooting Guides
  
  Posters and Literature
  
  Handbooks and Guides
  
  Calculators
  
  FAQs
  
  Blogs
  
  Diseases
  
  Whitepapers
  
  Antibody Concierge
  
  Webinars
- Tools
  Spectra Viewer
  
  Flow Cytometry Panel Builder
  
  Application
  ELISA
  
  Flow Cytometry
  
  Mass Cytometry (CyTOF)
  
  ICC
  
  IHC
  
  Western Blotting
  
  Interviews
  Faculty
  
  Postdoc
Resources
View Pathways
- By Research Area
  Apoptosis
  
  Autophagy
  
  Cancer
  
  Cell Biology
  
  Cellular Markers
  
  Epigenetics
  
  Epitope Tags
  
  Exosomes Research
  
  Hypoxia
  
  Immunology
  
  Microbiology
  
  Neuroscience
  
  Stem Cells
- By Pathway
  AKT
  
  Apoptosis
  
  Autophagy
  
  ERK1/2/5 Pathway
  
  Hedgehog
  
  Hippo
  
  JNK Pathway
  
  mTOR
  
  Necroptosis
  
  NFKB Signaling
  
  p38 Signaling
  
  TGF Beta
  
  TLR
Research Areas
Get Rewards
- Customer Care
  100% Guarantee
  
  Risk Free Testing
  
  Novus Rewards Program
  
  Publication Rewards Program
  
  New Lab Discount
  
  Scholarship
  
  Travel Grants
  
  Promotions
Customer Care
Novus Careers
- About us
  Careers
  
  Collaborations
  
  Events
  
  Novus Story
  
  Our Team
  
  Philanthropy
  
  Press Releases
  
  Why choose Novus
About Us
- Contact Us
  Customer Service
  
  Distributors
  
  Technical Support
Contact

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	Bioactivity
Format	Carrier-Free

Details of Functionality	Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED₅₀ for this effect is 5-20 ng/mL.
Source	E. coli-derived mouse Complement Component C5a protein Asn679-Arg755, with an N-terminal Met
Accession #	P06684
N-terminal Sequence	Met
Protein/Peptide Type	Recombinant Proteins
Gene	Hc
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note	<0.10 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS.

Publications using 2150-C5/CF	Applications	Species
W Xiong, L Jia, J Liang, Y Cai, Y Chen, Y Nie, J Jin, J Zhu Investigation into the anti-airway inflammatory role of the PI3Kgamma inhibitor JN-PK1: An in vitro and in vivo study International immunopharmacology, 2022-08-11;111(0):109102. 2022-08-11 [PMID: 35964410] (Cell Culture, Mouse)	Cell Culture	Mouse
M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022-07-14;19(1):182. 2022-07-14 [PMID: 35831910] (Bioassay, Mouse)	Bioassay	Mouse
L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions Cell Death & Disease, 2022-05-25;13(5):500. 2022-05-25 [PMID: 35614037] (Bioassay, Mouse)	Bioassay	Mouse
FM Consonni, A Bleve, MG Totaro, M Storto, P Kunderfran, A Termanini, F Pasqualini, C Alì, C Pandolfo, F Sgambellur, G Grazia, M Santinami, A Maurichi, M Milione, M Erreni, A Doni, M Fabbri, L Gribaldo, E Rulli, MP Soares, V Torri, R Mortarini, A Anichini, A Sica Heme catabolism by tumor-associated macrophages controls metastasis formation Nature Immunology, 2021-04-26;22(5):595-606. 2021-04-26 [PMID: 33903766] (Bioassay, Mouse)	Bioassay	Mouse
E van den Bo, B Ambrosy, M Horsthemke, S Walbaum, AC Bachg, N Wettschure, G Innamorati, TM Wilkie, PJ Hanley Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis J. Biol. Chem., 2020-04-24;0(0):. 2020-04-24 [PMID: 32332099] (Bioassay, Mouse)	Bioassay	Mouse
AB Schmider, NC Bauer, H Sunwoo, MD Godin, GE Ellis, JT Lee, PA Nigrovic, RJ Soberman Two and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils J. Biol. Chem., 2020-03-09;0(0):. 2020-03-09 [PMID: 32152223] (Bioassay, Mouse)	Bioassay	Mouse
BE Hsu, J Roy, J Mouhanna, RF Rayes, L Ramsay, S Tabariès, MG Annis, IR Watson, JD Spicer, S Costantino, PM Siegel C3a elicits unique migratory responses in immature low-density neutrophils Oncogene, 2020-02-04;0(0):. 2020-02-04 [PMID: 32020055] (Bioassay, Mouse)	Bioassay	Mouse
C Muth, A Hartmann, D Sepulveda-, M Glatzel, S Krasemann Phagocytosis of Apoptotic Cells Is Specifically Upregulated in ApoE4 Expressing Microglia in vitro Front Cell Neurosci, 2019-05-03;13(0):181. 2019-05-03 [PMID: 31130847] (Bioassay, Mouse)	Bioassay	Mouse
K Kobayakawa, Y Ohkawa, S Yoshizaki, T Tamaru, T Saito, K Kijima, K Yokota, M Hara, K Kubota, Y Matsumoto, K Harimaya, K Ozato, T Masuda, M Tsuda, T Tamura, K Inoue, VR Edgerton, Y Iwamoto, Y Nakashima, S Okada Macrophage centripetal migration drives spontaneous healing process after spinal cord injury Sci Adv, 2019-05-15;5(5):eaav5086. 2019-05-15 [PMID: 31106270] (In Vivo, Mouse)	In Vivo	Mouse
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019-03-14;9(1):4515. 2019-03-14 [PMID: 30872638] (Bioassay, Mouse)	Bioassay	Mouse
S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis Nat. Immunol., 2019-01-14;20(2):141-151. 2019-01-14 [PMID: 30643265] (Bioassay, Mouse)	Bioassay	Mouse
MA Crowley, O Delgado, A Will-Orreg, NM Buchanan, K Anderson, BD Jaffee, TP Dryja, SM Liao Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):940-951. 2018-02-01 [PMID: 29450541] (Bioassay, Mouse)	Bioassay	Mouse
CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):662-673. 2018-02-01 [PMID: 29392311] (Mouse)		Mouse
JB Herrmann, M Muensterma, L Strobel, A Schubert-U, TM Woodruff, SD Gray-Owen, A Klos, KO Johswich Complement C5a Receptor 1 Exacerbates the Pathophysiology of N.ï¿½meningitidis Sepsis and Is a Potential Target for Disease Treatment MBio, 2018-01-23;9(1):. 2018-01-23 [PMID: 29362231] (ELISA (Standard), Mouse)	ELISA (Standard)	Mouse
SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice Eur. J. Immunol., 2018-01-15;0(0):. 2018-01-15 [PMID: 29277896] (Bioassay)	Bioassay
Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg Sci Rep, 2017-06-15;7(1):3603. 2017-06-15 [PMID: 28620195] (Bioassay, Mouse)	Bioassay	Mouse
S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing PLoS ONE, 2017-06-14;12(6):e0179512. 2017-06-14 [PMID: 28614388] (Bioassay, Mouse)	Bioassay	Mouse
MM Markiewski, SK Vadrevu, SK Sharma, NK Chintala, S Ghouse, JH Cho, DP Fairlie, Y Paterson, A Astrinidis, M Karbownicz The Ribosomal Protein S19 Suppresses Antitumor Immune Responses via the Complement C5a Receptor 1 J. Immunol., 2017-02-22;198(7):2989-2999. 2017-02-22 [PMID: 28228558] (Bioassay, Mouse)	Bioassay	Mouse
The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1 J Neurosci, 2016-05-04;36(18):5055-70. 2016-05-04 [PMID: 27147658] (In Vivo, Mouse)	In Vivo	Mouse
Sexual dimorphism of liver metastasis by murine pancreatic neuroendocrine tumors is affected by expression of complement C5 Oncotarget, 2016-05-24;7(21):30585-96. 2016-05-24 [PMID: 27105526] (Bioassay, Mouse)	Bioassay	Mouse
Hillgruber C, Poppelmann B, Weishaupt C, Steingraber A, Wessel F, Berdel W, Gessner J, Ho-Tin-Noe B, Vestweber D, Goerge T Blocking neutrophil diapedesis prevents hemorrhage during thrombocytopenia. J Exp Med, 2015-07-13;212(8):1255-66. 2015-07-13 [PMID: 26169941] (Bioassay, Mouse)	Bioassay	Mouse
Yatime , Laure, Maasch , Christia, Hoehlig , Kai, Klussmann , Sven, Andersen , Gregers, Vater , Axel Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun, 2015-04-22;6(0):6481. 2015-04-22 [PMID: 25901944] (Bioassay, Mouse)	Bioassay	Mouse
Piao C, Cai L, Qiu S, Jia L, Song W, Du J Complement 5a Enhances Hepatic Metastases of Colon Cancer via Monocyte Chemoattractant Protein-1-mediated Inflammatory Cell Infiltration. J Biol Chem, 2015-03-04;290(17):10667-76. 2015-03-04 [PMID: 25739439] (Bioassay, Mouse)	Bioassay	Mouse
Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol, 2014-12-29;194(3):1164-8. 2014-12-29 [PMID: 25548218] (Western Blot, Mouse)	Western Blot	Mouse
Pazos M, Pirzai W, Yonker L, Morisseau C, Gronert K, Hurley B Distinct cellular sources of hepoxilin A3 and leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration. J Immunol, 2014-12-29;194(3):1304-15. 2014-12-29 [PMID: 25548217] (Bioassay, Mouse)	Bioassay	Mouse
Grailer J, Fattahi F, Dick R, Zetoune F, Ward P Cutting edge: critical role for C5aRs in the development of septic lymphopenia in mice. J Immunol, 2014-12-24;194(3):868-72. 2014-12-24 [PMID: 25539817] (Bioassay, Mouse)	Bioassay	Mouse
Vijayan S, Asare Y, Grommes J, Soehnlein O, Lutgens E, Shagdarsuren G, Togtokh A, Jacobs M, Fischer J, Bernhagen J, Weber C, Schober A, Shagdarsuren E High expression of C5L2 correlates with high proinflammatory cytokine expression in advanced human atherosclerotic plaques. Am J Pathol, 2014-05-09;184(7):2123-33. 2014-05-09 [PMID: 24819959] (Bioassay, Mouse)	Bioassay	Mouse
Kim J, Oh S, Ahn S, Chung D IFN-gamma-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils. Eur J Immunol, 2014-05-02;44(7):2025-35. 2014-05-02 [PMID: 24723363] (ELISA (Standard))	ELISA (Standard)
Hsu W, Yang F, Lin C, Hsieh S, Chen N C5L2 is required for C5a-triggered receptor internalization and ERK signaling. Cell Signal, 2014-03-12;26(7):1409-19. 2014-03-12 [PMID: 24631530] (Bioassay, Human, Mouse)	Bioassay	Human, Mouse
Bosmann , Markus, Grailer , Jamison, Ruemmler , Robert, Russkamp , Norman F, Zetoune , Firas S, Sarma , J Vidya, Standiford , Theodore, Ward , Peter A Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury. FASEB J, 2013-08-27;27(12):5010-21. 2013-08-27 [PMID: 23982144] (In Vivo, Mouse)	In Vivo	Mouse
Hoehlig , Kai, Maasch , Christia, Shushakova , Nelli, Buchner , Klaus, Huber-Lang , Markus, Purschke , Werner G, Vater , Axel, Klussmann , Sven A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther, 2013-07-26;21(12):2236-46. 2013-07-26 [PMID: 23887360] (Surface Plasmon Resonance)	Surface Plasmon Resonance
Bosmann M, Haggadone M, Zetoune F, Sarma J, Ward P The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation. Eur J Immunol, 2013-05-13;43(7):1907-13. 2013-05-13 [PMID: 23575697] (Bioassay, Mouse)	Bioassay	Mouse
Abe T, Hosur K, Hajishengallis E, Reis E, Ricklin D, Lambris J, Hajishengallis G Local complement-targeted intervention in periodontitis: proof-of-concept using a C5a receptor (CD88) antagonist. J Immunol, 2012-10-22;189(11):5442-8. 2012-10-22 [PMID: 23089394] (Bioassay, Mouse)	Bioassay	Mouse
Gunn L, Ding C, Liu M, Ma Y, Qi C, Cai Y, Hu X, Aggarwal D, Zhang H, Yan J Opposing roles for complement component C5a in tumor progression and the tumor microenvironment. J Immunol, 2012-08-22;189(6):2985-94. 2012-08-22 [PMID: 22914051] (Bioassay, Mouse)	Bioassay	Mouse
Heimbach L, Li Z, Berkowitz P The C5a Receptor on Mast Cells Is Critical for the Autoimmune Skin-blistering Disease Bullous Pemphigoid. J. Biol. Chem., 2011-03-10;286(17):15003-9. 2011-03-10 [PMID: 21393236] (Bioassay, Mouse)	Bioassay	Mouse
Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss. J. Immunol., 2010-12-13;186(2):869-77. 2010-12-13 [PMID: 21149611] (Bioassay, Mouse)	Bioassay	Mouse
Chen NJ, Mirtsos C, Suh D, Lu YC, Lin WJ, McKerlie C, Lee T, Baribault H, Tian H, Yeh WC C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature, 2007-02-25;446(7132):203-7. 2007-02-25 [PMID: 17322907] (In Vivo, Mouse)	In Vivo	Mouse
Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice. Immunol. Lett., 2007-01-30;109(1):56-63. 2007-01-30 [PMID: 17292973] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Show All 38 Publications. Collapse Publications.

Recombinant Mouse Complement Component C5a Protein, CF

Species Glossary

Images

Product Details

Order Details

Recombinant Mouse Complement Component C5a Protein, CF Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Recombinant Mouse Complement Component C5a Protein, CF

Background

Customers Who Viewed This Item Also Viewed...

Publications for Complement Component C5a (2150-C5/CF)(38)

Reviews for Complement Component C5a (2150-C5/CF) (0)

FAQs for Complement Component C5a (2150-C5/CF) (0)

Additional Complement Component C5a Products

Blogs on Complement Component C5a

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics

Catalog# & Formulation	Size	Price
Standard Formulation 2150-C5-025

Gene Symbol	Hc
Uniprot	P06684()