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Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Standard Formulation
1017-MP-025/CF
Carrier Free
1017-MP-025/CF
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Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived mouse CCL24/Eotaxin-2/MPIF-2 protein
Val27-Val119
Accession #
N-terminal Sequence
Val27
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl24
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
10.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
1017-MP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein

  • CCL24
  • chemokine (C-C motif) ligand 24
  • Ckb-6
  • Eosinophil chemotactic protein 2
  • Eotaxin-2
  • member 24
  • MPIF2
  • MPIF-2
  • MPIF2Myeloid progenitor inhibitory factor 2
  • MPIF-2Small-inducible cytokine A24
  • SCYA24CK-beta-6

Background

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to Eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val27 - Val119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile24 - Val119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).

  1. Zimmermann, N. et al. (2000) J. Immunol. 165:5839.
  2. Watanabe, K. et al. (2002) J. Immunol. 168:1911.
  3. Grzegorzewski, K.J. et al. (2001) Cytokine 13:209.

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Recombinant Mouse
CCL24/Eotaxin-2/MPIF- ...
1017-MP
Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein
Species: Mu
Applications: Bioactivity

Publications for CCL24/Eotaxin-2/MPIF-2 (1017-MP)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Ccl24
Uniprot