| Reactivity | MuSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 150-600 ng/mL. In the same assay, the ED50 of the aa 27-119 mouse Eotaxin-2 variant (R&D Systems, Catalog # 1017-MP) is 1-5 ng/mL. The aa 24-119 mouse Eotaxin-2 variant is about 150-fold less active than the aa 27-119 mouse Eotaxin-2 variant. |
| Source | E. coli-derived mouse CCL24/Eotaxin-2/MPIF-2 protein Ile24-Val119 |
| Accession # | |
| N-terminal Sequence | Ile24 |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | Ccl24 |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
|
| Theoretical MW | 10.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val 27 - Val 119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile 24 - Val 119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).
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