Home » Carboxypeptidase B1/CPB1 » Carboxypeptidase B1/CPB1 Proteins » Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

 (1 Publication)
 See All Related
Submit a Review

Images

 
There are currently no images for Carboxypeptidase B1/CPB1 (2898-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Novus Biologicals offers 100% Guarantee

Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived mouse Carboxypeptidase B1/CPB1 protein
Ser16-Tyr415, with a C-terminal 10-His tag
Accession # NP_083982
Accession #
N-terminal Sequence
Ser16
Protein/Peptide Type
Recombinant Enzymes
Gene
Cpb1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
43 kDa and 48 kDa, reducing conditions
Publications
Read Publication using 2898-ZN.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in MES and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Activation Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
  • Assay Buffer: 25 mM Tris, 0.1 M NaCl, pH 7.5
  • Recombinant Mouse Carboxypeptidase B1/CPB1 (rmCPB1) (Catalog # 2898-ZN)
  • Trypsin (Sigma, Catalog # T-1426)
  • AEBSF (Catalog # EI001), 100 mM stock in DMSO
  • Substrate: Hippuryl-Arg (Sigma, Catalog # H-2508), 25 mM in diH2O
  • 96-well Clear UV Plate (Corning, Catalog # 3635)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Activate rmCPB1 at 100 µg/mL with 1 µg/mL Trypsin in Activation Buffer.
  2. Incubate at room temperature for 30 minutes.
  3. Add AEBSF at a final concentration of 1 mM to stop reaction.
  4. Dilute activated rmCPB1 to 2 µg/mL in Assay Buffer.
  5. Dilute Substrate to 2 mM in Assay Buffer.
  6. In a plate, load 50 µL of 2 µg/mL rmCPB1, and include a Substrate Blank containing 50 µL Assay Buffer.
  7. Start the reaction by adding 50 µL of 2 mM Substrate into wells.
  8. Read in kinetic mode for 5 minutes at an absorbance of 254 nm.
  9. Calculate specific activity using the following formula:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard Hippuric acid (Sigma Catalog # 112003).

Per Well:
  • rmCPB1: 0.1 µg
  • Substrate: 1 mM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). The deduced amino acid sequence of mouse CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 108), and a mature chain (residues 109 to 415). The purified rmCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).

  1. Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 831, Academic Press, San Diego.

Customers Who Viewed This Item Also Viewed...

IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(675 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(820 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(304 Publications)
Species: Mu
Applications: ELISA
Adenine Nucleotide
Translocator 2 Antibo ...
NBP2-92630
Immunohistochemistry-Paraffin Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Western Blot Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(184 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(71 Publications)
Species: Hu
Applications: ELISA
Plasminogen
Antibody
NB600-930
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
(2 Reviews)
(6 Publications)
Species: Hu, Rt
Applications: ELISA, WB
Carboxypeptidase
B2/CPB2 Antibody [Unc ...
AF6036
Immunohistochemistry Carboxypeptidase B2/CPB2 Antibody [Unconjugated]
Western Blot Carboxypeptidase B2/CPB2 Antibody [Unconjugated]
Species: Hu
Applications: IHC, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Prothrombin
Antibody
NBP1-58268
Immunohistochemistry-Paraffin Prothrombin Antibody
Immunohistochemistry-Paraffin Prothrombin Antibody
Western Blot Prothrombin Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Serpin A3/alpha
1-Antichymotrypsin An ...
NBP2-52559
Western Blot Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
ELISA Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
Flow Cytometry Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
(1 Publication)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
Actin Gamma 1
Antibody
NB600-533
Western Blot Actin Gamma 1 Antibody
Western Blot Actin Gamma 1 Antibody
Western Blot Actin Gamma 1 Antibody
(6 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
actin, gamma 2
Antibody
NBP3-42341
Immunohistochemistry-Paraffin actin, gamma 2 Antibody
Immunohistochemistry-Paraffin actin, gamma 2 Antibody
Western Blot actin, gamma 2 Antibody
Species: Hu, Po, Rt
Applications: IHC, WB
FHL5 Antibody
NBP1-32660
Immunocytochemistry/ Immunofluorescence FHL5 Antibody
Immunohistochemistry-Paraffin FHL5 Antibody
Western Blot FHL5 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ACOT7 Antibody
NBP1-89278
Immunocytochemistry/ Immunofluorescence ACOT7 Antibody
Immunohistochemistry-Paraffin ACOT7 Antibody
Western Blot ACOT7 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Neutrophil
Elastase/ELA2 [Biotin
DY4517-05
N/A Neutrophil Elastase/ELA2 [Biotin]
(15 Publications)
Species: Mu
Applications: ELISA
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(25 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Serpin
C1/Antithrombin-III A ...
AF1267
Serpin C1/Antithrombin-III Antibody [Unconjugated]
(6 Publications)
Species: Hu
Applications: IP, Neut, WB
NDUFB6 Antibody
NBP1-92172
Immunohistochemistry-Paraffin NDUFB6 Antibody
Western Blot NDUFB6 Antibody
Immunocytochemistry/ Immunofluorescence NDUFB6 Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Myeloperoxidase/MPO An ...
AF3667
Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]
Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]
Immunocytochemistry Myeloperoxidase/MPO Antibody [Unconjugated]
(3 Reviews)
(168 Publications)
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB

Publications for Carboxypeptidase B1/CPB1 (2898-ZN)(1)

We have publications tested in 1 application: ELISA (Standard).


Filter By Application
ELISA (Standard)
(1)
All Applications
Filter By Species
All Species

Reviews for Carboxypeptidase B1/CPB1 (2898-ZN) (0)

There are no reviews for Carboxypeptidase B1/CPB1 (2898-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Carboxypeptidase B1/CPB1 (2898-ZN). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Additional Carboxypeptidase B1/CPB1 Products

Blogs on Carboxypeptidase B1/CPB1

There are no specific blogs for Carboxypeptidase B1/CPB1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol Cpb1
Uniprot