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Recombinant Human VEGF 121 (aa 207-327) Protein

 (16 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
4644-VS-010/CF
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Recombinant Human VEGF 121 (aa 207-327) Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.00-8.00 ng/mL.
Source
E. coli-derived human VEGF protein
Ala207-Arg327, with an N-terminal Met & Pro208-Arg327
Accession #
N-terminal Sequence
Met & Pro208
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
VEGFA
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
4644-VS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF 121 (aa 207-327) Protein

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cysteine-knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). VEGF121 is the only form that lacks a basic heparin-binding region and is freely diffusible (4). Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis (5). Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1; VEGF121 binding has also been reported (6). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (7, 8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 10).

  1. Leung, D.W. et al. (1989) Science 246:1306.
  2. Keck, P.J. et al. (1989) Science 246:1309.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Zelzer, E. et al. (2002) Development 129:1893.
  6. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  7. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  8. Thurston, G. (2002) J. Anat. 200:575.
  9. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  10. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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Publications for VEGF (4644-VS)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Hamster, N/A.

We have publications tested in 5 applications: Bioassay, ELISA Capture, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR), Western Blot.


Filter By Application
Bioassay
(12)
ELISA Capture
(1)
Surface Plasmon Resonance
(1)
Surface Plasmon Resonance (SPR)
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
Hamster
(1)
N/A
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 4644-VS Applications Species
Tzuri, N;Yegodayev, KM;Novoplansky, O;Elkabets, M;Aharoni, A;Papo, N; Developing a dual VEGF/PDL1 inhibitor based on high-affinity scFv heterodimers as an anti-cancer therapeutic strategy Scientific reports 2023-07-24 [PMID: 37488176] (Surface Plasmon Resonance (SPR), N/A) Surface Plasmon Resonance (SPR) N/A
Y Ichiyama, R Matsumoto, S Obata, O Sawada, Y Saishin, M Kakinoki, T Sawada, M Ohji Assessment of mouse VEGF neutralization by ranibizumab and aflibercept PLoS ONE, 2022-12-21;17(12):e0278951. 2022-12-21 [PMID: 36542626] (Western Blot, N/A) Western Blot N/A
S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance Cell Reports, 2021-08-17;36(7):109549. 2021-08-17 [PMID: 34407412] (Bioassay, Human) Bioassay Human
L Martin, P Bouvet, N Chounlamou, C Watrin, R Besançon, D Pinatel, D Meyronet, J Honnorat, A Buisson, PA Salin, C Meissirel VEGF counteracts amyloid-&amp;beta-induced synaptic dysfunction Cell Reports, 2021-05-11;35(6):109121. 2021-05-11 [PMID: 33979625] (Bioassay, ELISA Capture, Human) Bioassay, ELISA Capture Human
F Njau, N Shushakova, H Schenk, VC Wulfmeyer, R Bollin, J Menne, H Haller Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice PLoS ONE, 2020-01-14;15(1):e0218494. 2020-01-14 [PMID: 31935212] (Bioassay, Human) Bioassay Human
Ö Uslu, J Herold, S M Kanse VEGF-A-Cleavage by FSAP and Inhibition of Neo-Vascularization Cells, 2019-11-06;8(11):. 2019-11-06 [PMID: 31698750] (Bioassay, Human) Bioassay Human
C Abou Fayca, S Gazzeri, B Eymin A VEGF-A/SOX2/SRSF2 network controls VEGFR1 pre-mRNA alternative splicing in lung carcinoma cells Sci Rep, 2019-01-23;9(1):336. 2019-01-23 [PMID: 30674935] (Bioassay, Human) Bioassay Human
S Stephenson, MA Care, I Fan, A Zougman, DR Westhead, GM Doody, RM Tooze Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells J. Immunol., 2019-01-14;202(4):1287-1300. 2019-01-14 [PMID: 30642980] (Bioassay, Human) Bioassay Human
N Patel, S Able, D Allen, E Fokas, B Cornelisse, FV Gleeson, AL Harris, KA Vallis Monitoring response to anti-angiogenic mTOR inhibitor therapy in vivo using (111)In-bevacizumab EJNMMI Res, 2017-05-30;7(1):49. 2017-05-30 [PMID: 28560583] (Western Blot) Western Blot
S Kapur, AP Silverman, AZ Ye, N Papo, D Jindal, MS Blumenkran, JR Cochran Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis Bioeng Transl Med, 2017-02-17;2(1):81-91. 2017-02-17 [PMID: 28516164] (Bioassay, Human) Bioassay Human
Show All 16 Publications.

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FAQs for VEGF (4644-VS). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.
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Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.
Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.
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Bioinformatics

Gene Symbol VEGFA
Uniprot