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Recombinant Human TFF3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TFF3 Protein, CF Summary

Details of Functionality
Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF3 can effectively induce ERK1/2 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human TFF3 protein
Glu22-Phe80 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TFF3
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7-11 kDa, reducing conditions
Publications
Read Publication using
8294-TF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TFF3 Protein, CF

  • HITF, human intestinal trefoil factor10Intestinal trefoil factor
  • ITF
  • TFF3
  • TFI
  • trefoil factor 3 (intestinal)

Background

Trefoil Factor 3 (TFF3), also known as Intestinal Trefoil Factor (ITF) and P1.B, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF3 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2 (2, 3). TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach (4-8). Following secretion, TFF3 can be retained in the overlying mucous layer (7). TFF3 is also expressed by chondrocytes during bone development (9). Mature human TFF3 shares 76% amino acid sequence identity with mouse and rat TFF3. TFF3 is up-regulated in response to a range of gastrointestinal epithelial disruptions (8, 10). It promotes epithelial wound healing by inducing the migration of biliary, bronchial, and intestinal epithelial cells (5, 10-12). TFF3 up-regulation is associated with and enhances tumor cell invasion and metastasis (6, 13). It supports hypoxia-induced VEGF up-regulation in tumor cells and also promotes angiogenesis in non-tumor environments (14, 15). Over-expression of TFF3 in type 2 diabetic mouse liver has been shown to improve glucose tolerance and insulin sensitivity (16).
  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Albert, T.K. et al. (2010) J. Proteome Res. 9:3108.
  3. Yu, H. et al. (2011) PLoS ONE 6:e20334.
  4. LeSimple, P. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:296.
  5. Nozaki, I. et al. (2004) Am. J. Pathol. 165:1907.
  6. Ahmed, A.R.H. et al. (2012) Am. J. Pathol. 180:904.
  7. Podolsky, D.K. et al. (1993) J. Biol. Chem. 268:6694.
  8. Peitz, U. et al. (2004) Peptides 25:771.
  9. Bijelic, N. et al. (2013) Acta Histochem. 115:204.
  10. Paulsen, F.P. et al. (2008) J. Biol. Chem. 283:13418.
  11. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.
  12. Dignass, A. et al. (1994) J. Clin. Invest. 94:376.
  13. Rivat, C. et al. (2005) Cancer Res. 65:195.
  14. Rodrigues, S. et al. (2003) FASEB J. 17:7.
  15. Guleng, B. et al. (2012) Mol. Biol. Rep. 39:4127.
  16. Xue, Y. et al. (2013) PLoS ONE 8:e75240.

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Publications for TFF3 (8294-TF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol TFF3
Uniprot