Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Human PSGL-1 cDNA encodes 412 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (1). The ECD of human PSGL-1 contains 16 decameric repeats in the A allele (long form), which is expressed by 65-80% of the population. Alleles B and C contain 15 and 14 repeats, respectively, and mouse PSGL-1 contains 10-12 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Human PSGL-1 ECD shares less than 50% aa sequence identity with mouse, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-412) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2‑4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (LeX) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E-Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).

We have publications tested in 6 confirmed species: Human, Mouse, Akkermansia muciniphila, Bacteria, Bacteria - E. Coli, N/A.

We have publications tested in 11 applications: Antibody Production, Binding Assay, Bioassay, Blocking, ELISA Capture, ELISA Detection, Enzyme Assay, Enzyme Assay Substrate, Flow Cytometry, Surface Plasmon Resonance, Western Blot.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using 3345-PS	Applications	Species
Novobrantseva, T;Manfra, D;Ritter, J;Razlog, M;O'Nuallain, B;Zafari, M;Nowakowska, D;Basinski, S;Phennicie, RT;Nguyen, PA;Brehm, MA;Sazinsky, S;Feldman, I; Preclinical Efficacy of VTX-0811: A Humanized First-in-Class PSGL-1 mAb Targeting TAMs to Suppress Tumor Growth Cancers 2024-08-06 [PMID: 39199551] (Antibody Production, ELISA Capture, Mouse)	Antibody Production, ELISA Capture	Mouse
Ruzemaimaiti, D;Sun, H;Zhang, J;Xu, C;Chen, L;Yin, R;Zhao, J; Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand Carbohydrate polymers 2023-10-01 [PMID: 37364953] (ELISA Detection, N/A)	ELISA Detection	N/A
BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond The Journal of Biological Chemistry, 2022-08-30;0(0):102439. 2022-08-30 [PMID: 36049519] (Bioassay, Human)	Bioassay	Human
MS Saito, KC Zatta, PC Sathler, PS Furtado, N C O Miguel, FS Frattani, M Berger, V Lavayen, AR Pohlmann, SS Guterres Therapeutic implementation in arterial thrombosis with pulmonary administration of fucoidan microparticles containing acetylsalicylic acid International journal of pharmaceutics, 2022-05-25;622(0):121841. 2022-05-25 [PMID: 35623486] (Bioassay, Human)	Bioassay	Human
DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences The Journal of Biological Chemistry, 2022-04-09;0(0):101917. 2022-04-09 [PMID: 35405095] (Bioassay, Akkermansia muciniphila)	Bioassay	Akkermansia muciniphila
AB Yasunaga, ITS Li Quantification of fast molecular adhesion by fluorescence footprinting Science Advances, 2021-08-18;7(34):. 2021-08-18 [PMID: 34407937] (Bioassay, Human)	Bioassay	Human
FW Lam, CA Brown, C Valladolid, DC Emebo, TG Palzkill, MA Cruz The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium PLoS ONE, 2020-10-13;15(10):e0240164. 2020-10-13 [PMID: 33048962] (Bioassay, N/A)	Bioassay	N/A
N Mehta, S Maddineni, RL Kelly, RB Lee, SA Hunter, JL Silberstei, RA Parra Sper, CL Miller, A Rabe, L Labanieh, JR Cochran An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA Scientific Reports, 2020-09-16;10(1):15171. 2020-09-16 [PMID: 32938950] (Bioassay, Human)	Bioassay	Human
SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):. 2019-03-25 [PMID: 30910957] (Bioassay, Bacteria - E. Coli)	Bioassay	Bacteria - E. Coli
SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities J. Immunol., 2018-04-23;0(0):. 2018-04-23 [PMID: 29686052] (Bioassay, Human)	Bioassay	Human
FW Lam, Q Da, B Guillory, MA Cruz Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium J. Immunol., 2018-01-15;0(0):. 2018-01-15 [PMID: 29335256] (Blocking, Human)	Blocking	Human
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016-11-28;6(0):37953. 2016-11-28 [PMID: 27892504] (Bioassay)	Bioassay
Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry. J Virol, 2015-09-23;89(23):12084-95. 2015-09-23 [PMID: 26401034] (Bioassay, Human)	Bioassay	Human
Gutierrez D, Pardo M, Montero D, Onate A, Farfan M, Ruiz-Perez F, Del Canto F, Vidal R TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain. Infect Immun, 2015-02-23;83(5):1893-903. 2015-02-23 [PMID: 25712927] (Bioassay, Bacteria - E. Coli)	Bioassay	Bacteria - E. Coli
Ayala-Lujan , Jorge Lu, Vijayakumar , Vidhya, Gong , Mei, Smith , Rachel, Santiago , Araceli, Ruiz-Perez , Fernando Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE, 2014-09-24;9(9):e107920. 2014-09-24 [PMID: 25251283] (Enzyme Assay, Enzyme Assay Substrate, Bacteria, Human)	Enzyme Assay, Enzyme Assay Substrate	Bacteria, Human
Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling. J Immunol, 2014-01-15;192(4):1862-9. 2014-01-15 [PMID: 24431230] (Bioassay, Flow Cytometry, Human)	Bioassay, Flow Cytometry	Human
Banks , Jessica, Herman , Christin, Bailey , Ryan C Bromelain decreases neutrophil interactions with P-selectin, but not E-selectin, in vitro by proteolytic cleavage of P-selectin glycoprotein ligand-1. PLoS ONE, 2013-11-11;8(11):e78988. 2013-11-11 [PMID: 24244398] (Bioassay)	Bioassay
Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat. Immunol., 2010-03-07;11(4):328-34. 2010-03-07 [PMID: 20208538] (Binding Assay, Human)	Binding Assay	Human
Wu ZL, Ethen CM, Larson S, Prather B, Jiang W A versatile polyacrylamide gel electrophoresis based sulfotransferase assay. BMC Biotechnol., 2010-02-10;10(0):11. 2010-02-10 [PMID: 20146816] (Enzyme Assay, Human)	Enzyme Assay	Human
Baker HM, Basu I, Chung MC, Caradoc-Davies T, Fraser JD, Baker EN Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins. J. Mol. Biol., 2007-10-10;374(5):1298-308. 2007-10-10 [PMID: 17996251] (Western Blot)	Western Blot
Simonis D, Fritzsche J, Alban S, Bendas G Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition. Biochemistry, 2007-04-26;46(20):6156-64. 2007-04-26 [PMID: 17458940] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Show All 21 Publications. Collapse Publications.

Array 3345-PS

• PSGL-1/CD162 Antibodies
• PSGL-1/CD162 ELISA Kit
• PSGL-1/CD162 Lysate
• PSGL-1/CD162 Protein
• PSGL-1/CD162 Proteins and Enzymes
• PSGL-1/CD162 ELISA