Recombinant Human NM23-H2 His-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Non-metastatic Protein 23 Homolog 2 (NM23-H2), also known as Nucleoside diphosphate kinase B (NDPK-B), is a group 1 member of the NDPK family encoded by the NME2 (non metastatic cell) gene (1,2). NDPKs are well-known as housekeeping genes that maintain the balance of nucleoside triphosphate levels by transferring a phosphate group from a nucleoside triphosphate to nucleoside diphosphate. NM23-H2 is a cytosolic protein that forms active hexamers composed of a trimer of dimers (3). The active hexamer contains a "head" region with substrate binding and catalytic activity as well as a Kpn loop and C-terminal extensions that stabilize the structure (3). NM23-H2 (NDPK-B) shares significant homology with NM23-H1 (NDPK-A) at 88% identity (3). In spite of significant homology and catalytic function between these isoforms, the differing amino acids present on the lateral surface of the hexamers lead to specific interactions with other proteins and ultimately result in diverging cellular distribution and function in cells (2).  Both NM23-H2 and NM23-H1 have been reported to act as protein histidine kinases (4-7). NM23-H2 alone has been shown to reversibly histidine phosphorylate beta subunit of trimeric G proteins (4), the Kca3.1 potassium channel (5) and the TRPV5 calcium channel (6) implicating NM23-H2 to have a role in heart failure and activation of B and T cells. Additionally, NM23-H2 has specifically been shown to form a complex with the cystic fibrosis transmembrane conductance regulator (CFTR) (1) to regulate chloride channels. NM23-H2 knockout mice have a defect in potassium channel activation, cytokine production in T cells (8), and have been shown to decrease class switch recombination (CSR) in B cells during an immune response (9).

Array 10466-NM

• NM23-H2/NME2 Antibodies
• NM23-H2/NME2 Lysates
• NM23-H2/NME2 Proteins
• NM23-H2/NME2 Proteins and Enzyme