Recombinant Human Integrin alpha 2 beta 1 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit (1-3). It is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83-89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I-III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E‑cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8-10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non‑hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

We have publications tested in 2 confirmed species: Human, Bacteria - Peptostreptococcus anaerobius.

We have publications tested in 2 applications: Binding Assay, Bioassay.

Showing Publications 1 - 4 of 4.

Publications using 5698-A2	Applications	Species
C Zeltz, M Khalil, R Navab, MS Tsao Collagen Type XI Inhibits Lung Cancer-Associated Fibroblast Functions and Restrains the Integrin Binding Site Availability on Collagen Type I Matrix International Journal of Molecular Sciences, 2022-10-03;23(19):. 2022-10-03 [PMID: 36233024] (Bioassay, Human)	Bioassay	Human
X Long, CC Wong, L Tong, ESH Chu, C Ho Szeto, MYY Go, OO Coker, AWH Chan, FKL Chan, JJY Sung, J Yu Peptostreptococcus anaerobius promotes colorectal carcinogenesis and modulates tumour immunity Nat Microbiol, 2019-09-09;0(0):. 2019-09-09 [PMID: 31501538] (Bioassay, Bacteria - Peptostreptococcus anaerobius)	Bioassay	Bacteria - Peptostreptococcus anaerobius
Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013-11-18;8(11):e81165. 2013-11-18 [PMID: 24260554] (Binding Assay)	Binding Assay
Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells. J Biol Chem, 2012-07-25;287(39):32981-92. 2012-07-25 [PMID: 22833667] (Bioassay, Human)	Bioassay	Human

Array 5698-A2

• Integrin alpha 2 beta 1 Antibodies
• Integrin alpha 2 beta 1 Proteins and Enzymes