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Recombinant Human IL-13 Protein, CF

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Recombinant human IL-13 (213-ILB/CF) stimulates proliferation in the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.5-2.5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-13 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human IL-13 protein
Gly21-Asn132
Accession #
N-terminal Sequence
Gly21
Protein/Peptide Type
Recombinant Proteins
Gene
IL13
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Publications
Read Publications using
213-ILB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-13 Protein, CF

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells (1‑3). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (4). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑regulation of CD23/Fc epsilon RII (1, 5). IL-13 binds with low affinity to the transmembrane IL-13 R alpha 1 which then forms a signaling complex with the transmembrane IL-4 R alpha (6‑8). This high affinity receptor complex also functions as the type 2 IL-4 receptor (6, 7). IL‑13 R alpha 2 inhibits responses to both IL-13 and IL-4. It binds IL-13 with high affinity (9, 10) and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex (11, 12). It also blocks signaling through IL-4-occupied IL-13 R alpha 1/IL-4 R alpha receptor complexes (12, 13). In addition, IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis (14‑16).
  1. Saggini, A. et al. (2011) Int. J. Immunopathol. Pharmacol. 24:305.
  2. Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.
  3. Liang, H.E. et al. (2012) Nat. Immunol. 13:58.
  4. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  5. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  6. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Chen, W. et al. (2009) J. Immunol. 183:7870.
  10. Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.
  11. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  12. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  13. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  14. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
  15. Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.
  16. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

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Publications for IL-13 (213-ILB/CF)(120)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), Neutralization, Polarization, Stimulation.


Filter By Application
Bioassay
(97)
Cell Culture
(16)
Differentiation
(2)
ELISA (Standard)
(1)
Neutralization
(1)
Polarization
(2)
Stimulation
(1)
All Applications
Filter By Species
Human
(117)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 120. Show All 120 Publications.
Publications using 213-ILB/CF Applications Species
Ma, HY;Li, Q;Wong, WR;N'Diaye, EN;Caplazi, P;Bender, H;Huang, Z;Arlantico, A;Jeet, S;Wong, A;Emson, C;Brightbill, H;Tam, L;Newman, R;Roose-Girma, M;Sandoval, W;Ding, N; LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung Science advances 2023-05-26 [PMID: 37235663] (Bioassay, Human) Bioassay Human
L Lerouge, M Gries, A Chateau, J Daouk, F Lux, P Rocchi, J Cedervall, AK Olsson, O Tillement, C Frochot, S Acherar, N Thomas, M Barberi-He Targeting Glioblastoma-Associated Macrophages for Photodynamic Therapy Using AGuIX�-Design Nanoparticles Pharmaceutics, 2023-03-20;15(3):. 2023-03-20 [PMID: 36986856] (Bioassay, Human) Bioassay Human
K Long, J Liu, S Shen, M Thong, D Wang, N Chen Light-sheet laser speckle imaging for cilia motility assessment Computational and structural biotechnology journal, 2023-02-20;21(0):1661-1669. 2023-02-20 [PMID: 36874161] (Bioassay, Human) Bioassay Human
CW Ku, J Yang, HY Tan, JKY Chan, YH Lee Decreased Innate Migration of Pro-Inflammatory M1 Macrophages through the Mesothelial Membrane Is Affected by Ceramide Kinase and Ceramide 1-P International Journal of Molecular Sciences, 2022-12-15;23(24):. 2022-12-15 [PMID: 36555618] (Polarization, Human) Polarization Human
R Akter, K Kim, HY Kwon, Y Kim, YW Eom, HM Cho, MY Cho EMR1/ADGRE1 Expression in Cancer Cells Upregulated by Tumor-Associated Macrophages Is Related to Poor Prognosis in Colorectal Cancer Biomedicines, 2022-12-02;10(12):. 2022-12-02 [PMID: 36551877] (Cell Culture, Human) Cell Culture Human
FH Wang, CY Hsieh, CI Shen, CH Chuang, YH Chung, CC Kuo, KD Lee, CL Lin, HL Su Induction of type II collagen expression in M2 macrophages derived from peripheral blood mononuclear cells Scientific Reports, 2022-12-15;12(1):21663. 2022-12-15 [PMID: 36522405] (Bioassay, Human) Bioassay Human
A Mentxaka, J Gómez-Ambr, B Ramírez, A Rodríguez, S Becerril, G Neira, V Valentí, R Moncada, C Silva, X Unamuno, JA Cienfuegos, J Escalada, G Frühbeck, V Catalán Netrin-1 Promotes Visceral Adipose Tissue Inflammation in Obesity and Is Associated with Insulin Resistance Nutrients, 2022-10-18;14(20):. 2022-10-18 [PMID: 36297056] (Cell Culture, Human) Cell Culture Human
F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature Communications, 2022-10-11;13(1):5956. 2022-10-11 [PMID: 36220814] (Bioassay, Human) Bioassay Human
H Cho, HY Kwon, A Sharma, SH Lee, X Liu, N Miyamoto, JJ Kim, SH Im, NY Kang, YT Chang Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target Nature Communications, 2022-10-10;13(1):5974. 2022-10-10 [PMID: 36216803] (Bioassay, Human) Bioassay Human
P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 2022-09-27;40(13):111439. 2022-09-27 [PMID: 36170836] (Bioassay, Human) Bioassay Human
Show All 120 Publications.

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Bioinformatics

Gene Symbol IL13
Uniprot