Recombinant Human G-CSF Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma . Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G_o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo, SDS-Page.

Showing Publications 1 - 10 of 56. Show All 56 Publications. Collapse Publications.

Publications using 214-CS	Applications	Species
Paul, SK;Oshima, M;Patil, A;Sone, M;Kato, H;Maezawa, Y;Kaneko, H;Fukuyo, M;Rahmutulla, B;Ouchi, Y;Tsujimura, K;Nakanishi, M;Kaneda, A;Iwama, A;Yokote, K;Eto, K;Takayama, N; Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model Nature communications 2024-06-10 [PMID: 38858384] (Bioassay, Human)	Bioassay	Human
Payen, SH;Adhikari, K;Petereit, J;Uppal, T;Rossetto, CC;Verma, SC; SARS-CoV-2 superinfection in CD14+ monocytes with latent human cytomegalovirus (HCMV) promotes inflammatory cascade Virus research 2024-04-22 [PMID: 38642618] (Bioassay, Human)	Bioassay	Human
Bleuzé, M;Lavoie, JP;Bédard, C;Gottschalk, M;Segura, M; Encapsulated Streptococcus suis impairs optimal neutrophil functions which are not rescued by priming with colony-stimulating factors PloS one 2024-01-23 [PMID: 38261585] (Bioassay, Porcine)	Bioassay	Porcine
Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F; c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities Cancers 2023-05-05 [PMID: 37174093]
JR James, J Curd, JC Ashworth, M Abuhantash, M Grundy, CH Seedhouse, KP Arkill, AJ Wright, CLR Merry, A Thompson Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML International Journal of Molecular Sciences, 2023-02-20;24(4):. 2023-02-20 [PMID: 36835644] (Bioassay, Human)	Bioassay	Human
CM Biggs, A Cordeiro-S, SV Prykhozhij, AP Deveau, Y Lin, KL Del Bel, F Orben, RJ Ragotte, A Saferali, S Mostafavi, L Dinh, D Dai, KG Weinacht, K Dobbs, L Ott de Bru, M Sharma, K Tsai, JJ Priatel, RA Schreiber, J Rozmus, MC Hosking, KE Shopsowitz, ML McKinnon, S Vercautere, M Seear, LD Notarangel, FC Lynn, JN Berman, SE Turvey Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammation JCI Insight, 2022-12-22;7(24):. 2022-12-22 [PMID: 36546480] (Cell Culture, Human)	Cell Culture	Human
JH Kim, JH Hong, KW Kang, BH Lee, Y Park, BS Kim Generation of the human pluripotent stem cell lines KUMi005-A from a patients with multiple myeloma Stem Cell Research, 2022-10-13;65(0):102939. 2022-10-13 [PMID: 36332466] (Bioassay, Human)	Bioassay	Human
YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Bioassay, Human)	Bioassay	Human
JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of human induced iPSC KUMi003-A from acute promyelocytic leukemia (APL) M3 Oncogene, 2022-07-09;63(0):102861. 2022-07-09 [PMID: 35905670] (Bioassay, Human)	Bioassay	Human
BA Krishna, AB Wass, EA Murphy, CM O'Connor Design of a US28 ORF Deletion Virus in a Temperature-Sensitive Cytomegalovirus Strain Fails to Promote Lytic Replication in Hematopoietic Cells Viruses, 2022-06-12;14(6):. 2022-06-12 [PMID: 35746751] (Bioassay, Human)	Bioassay	Human
JH Hong, JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma Stem Cell Research, 2022-03-28;61(0):102767. 2022-03-28 [PMID: 35397398] (Bioassay, Human)	Bioassay	Human
JH Kim, KW Kang, BH Lee, Y Park, H Jung Sung, BS Kim A human pluripotent stem cell line KUMi004-A generated from a patient with chronic lymphocytic leukemia Stem Cell Research, 2022-01-15;60(0):102668. 2022-01-15 [PMID: 35066240] (Bioassay, Human)	Bioassay	Human
JH Kim, KW Kang, BH Lee, Y Park, BS Kim Generation of induced pluripotent stem cell line KUMi002-A with normal karyotype from a patient with Philadelphia chromosome-positive chronic myeloid leukemia Stem Cell Research, 2021-07-12;55(0):102465. 2021-07-12 [PMID: 34303284] (Bioassay, Human)	Bioassay	Human
J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (Human)		Human
SJ Lee, JH Kim, KW Kang, BH Lee, BS Kim Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation Stem Cell Res, 2020-10-15;49(0):102030. 2020-10-15 [PMID: 33142253] (Cell Culture, Human)	Cell Culture	Human
SJ Lee, JH Kim, KW Kang, Y Park, BS Kim Supporting data on enhanced reprogramming of human CD34+ hematopoietic stem cells to induced pluripotent stem cells using human placenta-derived cell conditioned medium Data Brief, 2020-08-06;32(0):106140. 2020-08-06 [PMID: 32904343] (Bioassay, Human)	Bioassay	Human
BA Krishna, AB Wass, CM O'Connor Activator protein-1 transactivation of the major immediate early locus is a determinant of cytomegalovirus reactivation from latency Proc. Natl. Acad. Sci. U.S.A., 2020-08-11;117(34):20860-20867. 2020-08-11 [PMID: 32788362] (Bioassay, Human)	Bioassay	Human
SJ Lee, KW Kang, JH Kim, Y Park, BS Kim Generation of an induced pluripotent stem cell line KUMCi001-A from CD34+ bone marrow cells of a patient with acute lymphoblastic leukemia using human placenta-derived cell conditioned medium Stem Cell Res, 2020-07-13;47(0):101913. 2020-07-13 [PMID: 32707488] (Cell Culture, Human)	Cell Culture	Human
KW Kang, SJ Lee, JH Kim, BH Lee, SJ Kim, Y Park, BS Kim Etoposide-mediated interleukin-8 secretion from bone marrow stromal cells induces hematopoietic stem cell mobilization BMC Cancer, 2020-07-02;20(1):619. 2020-07-02 [PMID: 32615949] (Bioassay, Human)	Bioassay	Human
H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria Int J Mol Sci, 2020-06-15;21(12):. 2020-06-15 [PMID: 32549211] (Bioassay, Human)	Bioassay	Human
J Liu, S Xavy, S Mihardja, S Chen, K Sompalli, D Feng, TS Choi, B Agoram, R Majeti, IL Weissman, JP Volkmer Targeting macrophage checkpoint inhibitor SIRPa for anticancer therapy JCI Insight, 2020-06-18;0(0):. 2020-06-18 [PMID: 32427583] (Bioassay, Human)	Bioassay	Human
M Wakamatsu, Y Okuno, N Murakami, S Miwata, H Kitazawa, K Narita, S Kataoka, D Ichikawa, M Hamada, R Taniguchi, K Suzuki, N Kawashima, E Nishikawa, A Narita, N Nishio, S Kojima, H Muramatsu, Y Takahashi Detection of subclonal SETBP1 and JAK3 mutations in juvenile myelomonocytic leukemia using droplet digital PCR Leukemia, 2020-04-20;0(0):. 2020-04-20 [PMID: 32307441] (Bioassay, Human)	Bioassay	Human
M Nasri, M Ritter, P Mir, B Dannenmann, N Aghaallaei, D Amend, V Makaryan, Y Xu, B Fletcher, R Bernhard, I Steiert, K Hahnel, J Berger, I Koch, B Sailer, K Hipp, C Zeidler, M Klimiankou, B Bajoghli, DC Dale, K Welte, J Skokowa CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients Haematologica, 2019-06-27;0(0):. 2019-06-27 [PMID: 31248972] (Bioassay, Human)	Bioassay	Human
T Okumura, Y Horie, CY Lai, HT Lin, H Shoda, B Natsumoto, K Fujio, E Kumaki, T Okano, S Ono, K Tanita, T Morio, H Kanegane, H Hasegawa, F Mizoguchi, K Kawahata, H Kohsaka, H Moritake, H Nunoi, H Waki, SI Tamaru, T Sasako, T Yamauchi, T Kadowaki, H Tanaka, S Kitanaka, K Nishimura, M Ohtaka, M Nakanishi, M Otsu Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector Stem Cell Res Ther, 2019-06-24;10(1):185. 2019-06-24 [PMID: 31234949] (Bioassay, Human)	Bioassay	Human
M Ruiz-Gutie, ÖV Bölükba??, G Alexe, AG Kotini, K Ballotti, CE Joyce, DW Russell, K Stegmaier, K Myers, CD Novina, EP Papapetrou, A Shimamura Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells JCI Insight, 2019-04-30;5(0):. 2019-04-30 [PMID: 31039138] (Differentiation, Differentiation, Human)	Differentiation, Differentiation	Human
S Zheng, E Papalexi, A Butler, W Stephenson, R Satija Molecular transitions in early progenitors during human cord blood hematopoiesis Mol. Syst. Biol., 2018-03-15;14(3):e8041. 2018-03-15 [PMID: 29545397] (Bioassay, Human)	Bioassay	Human
H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise Mol. Cancer Ther., 2017-05-18;0(0):. 2017-05-18 [PMID: 28522588] (Bioassay, Human)	Bioassay	Human
MC Lee, JA McCubbin, AD Christense, DP Poole, P Rajasekhar, T Lieu, NW Bunnett, S Garcia-Car, A Erickson, SM Brierley, R Saleh, A Achuthan, AJ Fleetwood, RL Anderson, JA Hamilton, AD Cook G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease J. Immunol, 2017-03-20;0(0):. 2017-03-20 [PMID: 28320832] (In Vivo, Mouse)	In Vivo	Mouse
Thomas Müller Differentiation of induced pluripotent stem cell-derived neutrophil granulocytes from common marmoset monkey (Callithrix jacchus) Transfusion, 2016-11-25;0(0):. 2016-11-25 [PMID: 27888517] (Bioassay, Human)	Bioassay	Human
Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway Cell Biosci, 2016-08-30;6(1):50. 2016-08-30 [PMID: 27583127] (Bioassay, Human)	Bioassay	Human
Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion Stem Cells, 2016-05-27;0(0):. 2016-05-27 [PMID: 27145382] (Bioassay, Human)	Bioassay	Human
Wang F, Gao J, Malisani A, Xi X, Han W, Wan X Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis. BMC Biotechnol, 2015-11-16;15(0):105. 2015-11-16 [PMID: 26572487] (Bioassay, Mouse)	Bioassay	Mouse
Shima T, Iwasaki H, Yamauchi T, Kadowaki M, Kiyosuke M, Mochimaru T, Takenaka K, Miyamoto T, Akashi K, Teshima T Preserved in vivo reconstitution ability of PBSCs cryopreserved for a decade at -80 degrees C. Bone Marrow Transplant, 2015-06-22;50(9):1195-200. 2015-06-22 [PMID: 26098951] (Bioassay, Human)	Bioassay	Human
Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML). PLoS ONE, 2015-04-30;10(4):e0122983. 2015-04-30 [PMID: 25928216] (Bioassay, Human)	Bioassay	Human
Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells. Stem Cells, 2015-04-01;33(4):1142-52. 2015-04-01 [PMID: 25639500] (Bioassay, Human)	Bioassay	Human
Purushothaman P, Thakker S, Verma S Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells. J Virol, 2014-12-31;89(6):3093-111. 2014-12-31 [PMID: 25552714] (Bioassay, Human)	Bioassay	Human
Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice. Mol Ther, 2014-10-20;23(2):339-51. 2014-10-20 [PMID: 25327179] (Bioassay, Mouse)	Bioassay	Mouse
Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N Granulocyte-Colony Stimulating Factor related pathways tested on an endometrial ex-vivo model. PLoS ONE, 2014-10-02;9(9):e102286. 2014-10-02 [PMID: 25275446] (Bioassay, Human)	Bioassay	Human
Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling. Cancer Res, 2014-09-24;74(23):6806-19. 2014-09-24 [PMID: 25252914] (Bioassay, Human)	Bioassay	Human
Umashankar , Mahadeva, Rak , Michael, Bughio , Farah, Zagallo , Patricia, Caviness , Katie, Goodrum , Felicia Antagonistic determinants controlling replicative and latent states of human cytomegalovirus infection. J Virol, 2014-03-12;88(11):5987-6002. 2014-03-12 [PMID: 24623432] (Bioassay, Human)	Bioassay	Human
Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood, 2013-12-18;123(8):1239-49. 2013-12-18 [PMID: 24352879] (Bioassay, Human)	Bioassay	Human
Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa. J Invest Dermatol, 2013-12-06;134(5):1246-54. 2013-12-06 [PMID: 24317394] (Bioassay, Human)	Bioassay	Human
Xin , Hong, Lu , Rongze, Lee , Heehyoun, Zhang , Wang, Zhang , Chunyan, Deng , Jiehui, Liu , Yong, Shen , Shudan, Wagner , Kay-Uwe, Forman , Stephen, Jove , Richard, Yu , Hua G-protein-coupled receptor agonist BV8/prokineticin-2 and STAT3 protein form a feed-forward loop in both normal and malignant myeloid cells. J Biol Chem, 2013-04-02;288(19):13842-9. 2013-04-02 [PMID: 23548897] (Bioassay, Human)	Bioassay	Human
Ichii , Michiko, Frank , Mark Bar, Iozzo , Renato V, Kincade , Paul W The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 2011-11-23;119(7):1683-92. 2011-11-23 [PMID: 22117039] (Bioassay, Human)	Bioassay	Human
Torrano V, Procter J, Cardus P ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor. Blood, 2011-09-07;118(18):4910-8. 2011-09-07 [PMID: 21900195] (Bioassay, Human)	Bioassay	Human
Niwa A, Heike T, Umeda K A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011-07-27;6(7):e22261. 2011-07-27 [PMID: 21818303] (Bioassay, Human)	Bioassay	Human
Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome). Blood, 2010-10-29;117(3):839-47. 2010-10-29 [PMID: 21037085] (Bioassay, Human)	Bioassay	Human
Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock? Crit. Care Med., 2008-09-01;36(9):2583-9. 2008-09-01 [PMID: 18679115] (Bioassay, Human)	Bioassay	Human
Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008-05-27;36(8):1035-46. 2008-05-27 [PMID: 18504067] (Bioassay, Human)	Bioassay	Human
Ramachandran R, Morice AH, Compton SJ Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41. 2006-02-23 [PMID: 16498082] (ELISA (Standard))	ELISA (Standard)
Shao H, Xu X, Jing N, Tweardy DJ Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744. J. Immunol., 2006-03-01;176(5):2933-41. 2006-03-01 [PMID: 16493051] (Bioassay, Human)	Bioassay	Human
Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases. Blood, 2004-09-09;105(2):830-7. 2004-09-09 [PMID: 15358625] (Bioassay, Human)	Bioassay	Human
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R IL-22 increases the innate immunity of tissues. Immunity, 2004-08-01;21(2):241-54. 2004-08-01 [PMID: 15308104] (Bioassay, Human)	Bioassay	Human
Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein. Exp. Hematol., 2004-05-01;32(5):441-9. 2004-05-01 [PMID: 15145212] (Bioassay, Mouse)	Bioassay	Mouse
Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis. Blood, 2003-09-25;103(3):1089-98. 2003-09-25 [PMID: 14512323] (SDS-Page, Human)	SDS-Page	Human
Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman R, Schuler G Proliferating dendritic cell progenitors in human blood. J Exp Med, 1994-07-01;180(1):83-93. 1994-07-01 [PMID: 8006603] (Bioassay, Human)	Bioassay	Human
Show All 56 Publications. Collapse Publications.

Array 214-CS

• G-CSF Antibodies
• G-CSF Antibody Pair
• G-CSF ELISA Kit
• G-CSF Lysates
• G-CSF Proteins
• G-CSF ELISAs
• G-CSF Proteins and Enzymes