Novus Biologicals products are now on bio-techne.com

Recombinant Human FGF-9 Protein, CF

Images

 
Image 1 of 1
(
Enlarge)
Recombinant human FGF-9 (273-F9/CF) induces proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 1-5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

  • Size / Catalog#QtyPrice
    273-F9-025/CF
    25 ug
    $318.00
    1-3 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Recombinant Human FGF-9 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1-5 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human FGF-9 protein
Met1-Ser208 and Ala2-Ser208
Accession #
N-terminal Sequence
Met1 & Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
FGF9
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 22-29 kDa, reducing conditions
Publications
Read Publications using
273-F9/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-9 Protein, CF

  • FGF9
  • FGF-9
  • fibroblast growth factor 9 (glia-activating factor)
  • Fibroblast growth factor 9
  • GAF
  • glia-activating factor
  • HBFG-9
  • HBGF-9
  • Heparin-binding growth factor 9
  • MGC119914
  • MGC119915
  • SYNS3

Background

FGF-9 (fibroblast growth factor-9), also called HBGF-9 (heparin-binding growth factor-9) and GAF (glia-activating factor), is an approximately 26 kDa secreted glycoprotein of the FGF family (1-3). FGFs exhibit heparin-dependent regulation of cell proliferation, differentiation, and function, and are characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a beta -trefoil structure (1). FGF-9, -16 and -20 form a subfamily that shares 65-71% aa sequence identity, binds FGF R3 (IIIb), and are efficiently secreted despite having an uncleavable, bipartite signal sequence (1-3). Secreted human FGF-9 is a 205-207 aa protein that lacks the N-terminal 1-3 aa and shares >98% sequence identity with mouse, rat, equine, porcine and bovine FGF-9. In addition to FGF R3 (IIIb), FGF-9 binding to the IIIc splice forms of FGF R1, R2 and R3 are variably reported (3-5). An unusual constitutive dimerization of FGF-9 buries receptor interaction sites which lowers its activity, and increases heparin affinity which inhibits diffusion (4-6). A spontaneous mouse mutant, Eks, interferes with dimerization, resulting monomeric, diffusible FGF-9 that causes elbow and knee synostoses (joint fusions) due to FGF-9 misexpression in developing joints (6). In humans, FGF-9 mutations that lower receptor binding cause multiple synostoses syndrome (SYNS) (7). Expression in brain and kidney are reported in the adult rat (2, 8). In the mouse embryo the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes (1, 6-11). Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton (1, 10, 11). Altered FGF-9 expression or function is reported in human colon, endometrial, and ovarian cancers, correlating with progression, invasiveness, and survival (12-15).

  1. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  2. Miyamoto, M. et al. (1993) Mol. Cell. Biol. 13:4251.
  3. Santos-Ocampo, S. et al. (1996) J. Biol. Chem. 271:1726.
  4. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  5. Plotnikov, A.N. et al. (2001) J. Biol. Chem. 276:4322.
  6. Harada, M. et al. (2009) Nat. Genet. 41:289.
  7. Wu, X.L. et al. (2009) Am. J. Hum. Genet. 85:53.
  8. Colvin, J.S. et al. (1999) Dev. Dyn. 216:72.
  9. Lin, Y. et al. (2009) Dev. Biol. 329:44.
  10. Hung, I.H. et al. (2007) Dev. Biol. 307:300.
  11. Colvin, J.S. et al. (2001) Dev. Dyn 128:2095.
  12. Krejci, P. et al. (2009) Hum. Mutat. 30:1245.
  13. Leushacke, M. et al. (2011) PLoS ONE 6:e23381.
  14. Hendrix, N.D. et al. (2006) Cancer Res. 66:1354.
  15. Abdel-Rahman, W.M. et al. (2008) Hum. Mutat. 29:390.

Customers Who Viewed This Item Also Viewed...

233-FB
Species: Hu
Applications: BA
H00002263-M01
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information


Product PDFs

  PDF SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human FGF-9 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF9
Uniprot