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Recombinant Human DLL1 His-tag Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human DLL1 His-tag Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.25-1.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human DLL1 protein
Human DLL1
(Ser22-Gly540) &
(His152-Gly540)
Accession # AAG09716		 DI 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser22 & His152
Protein/Peptide Type
Recombinant Proteins
Gene
DLL1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
57 kDa and 43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa and 55 kDa, reducing conditions
Publications
Read Publications using
1818-DL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DLL1 His-tag Protein, CF

  • delta (Drosophila)-like 1
  • Delta 1
  • Delta
  • Delta1
  • delta-like 1 (Drosophila)
  • delta-like protein 1
  • DL1
  • DLL1
  • Drosophila Delta homolog 1
  • H-Delta-1

Background

Delta-like protein 1 (DLL1) is a 90 - 100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6 - 11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

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Publications for DLL1 (1818-DL)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(2)
Bioassay
(6)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using 1818-DL Applications Species
Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I; A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells Stem cell reports 2023-07-04 [PMID: 37451260] (Bioassay, Human) Bioassay Human
B Bohl, A Jabali, J Ladewig, P Koch Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells Science Advances, 2022-02-11;8(6):eabl5792. 2022-02-11 [PMID: 35148180] (Bioassay, Human) Bioassay Human
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Bioassay, Human) Bioassay Human
M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells Oncogene, 2019-10-15;0(0):. 2019-10-15 [PMID: 31616059] (Bioassay, Human) Bioassay Human
R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells J. Leukoc. Biol., 2018-10-08;105(1):101-111. 2018-10-08 [PMID: 30296338] (Binding Assay, Human) Binding Assay Human
Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat Immunol, 2012-10-21;13(12):1213-21. 2012-10-21 [PMID: 23086448] (Binding Assay) Binding Assay
Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol, 2012-07-16;181(3):1099-111. 2012-07-16 [PMID: 22809957] (Bioassay, Human) Bioassay Human
Pang RT, Leung CO, Ye TM, Liu W, Chiu PC, Lam KK, Lee KF, Yeung WS MicroRNA-34a suppresses invasion through downregulation of Notch1 and Jagged1 in cervical carcinoma and choriocarcinoma cells. Carcinogenesis, 2010-03-29;31(6):1037-44. 2010-03-29 [PMID: 20351093] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol DLL1
Uniprot