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DMBT1/GP340 Antibody (548012)

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Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse DMBT1 Monoclonal Antibody (Catalog # MAB59151) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DMBT1 at approximately 210 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse DMBT1 Monoclonal Antibody (Catalog # MAB59151) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
Cosmc deficiency results in accumulation of zymogen granules and pancreas atrophy in aged mice.a Representative TEM and brightfield micrographs of pancreas sections from fasting WT and Cosmc-KO adult mice. Zymogen ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
548012
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB59151-SP
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DMBT1/GP340 Antibody (548012) Summary

Immunogen
E.coli-derived recombinant mouse DMBT1
Trp215-Gly420
Accession # Q60997
Specificity
Detects mouse DMBT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD163 or recombinant human SRCRB4D is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
DMBT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB59151 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DMBT1/GP340 Antibody (548012)

  • CRP-ductin
  • deleted in malignant brain tumors 1 protein
  • deleted in malignant brain tumors 1
  • DMBT1
  • Ebnerin
  • FLJ61058
  • Glycoprotein 340
  • GP340
  • gp-340
  • GP340muclin
  • Hensin
  • MGC164738
  • Muclin
  • SAG
  • Salivary agglutinin
  • Surfactant pulmonary-associated D-binding protein
  • Vomeroglandin

Background

 

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DMBT1/GP340
Antibody (548012)
MAB59151
DMBT1/GP340 Antibody (548012)
Species: Mu
Applications: WB

Publications for DMBT1/GP340 Antibody (MAB59151)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
(2)
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Bioinformatics

Gene Symbol DMBT1
Uniprot