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Lunatic Fringe Antibody

Images

 
Western Blot: Lunatic Fringe Antibody [NBP2-39097] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Lunatic Fringe Antibody [NBP2-39097] - Staining of human liver shows very weak granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Lunatic Fringe Antibody [NBP2-39097] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Lunatic Fringe Antibody [NBP2-39097] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: Lunatic Fringe Antibody [NBP2-39097] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Lunatic Fringe Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: TKKFHRARLDLLLETWISRHKEMTFIFTDGEDEALARHTGNVVITNCSAAH
Predicted Species
Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LFNG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Lunatic Fringe Protein (NBP2-39097PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lunatic Fringe Antibody

  • beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
  • EC 2.4.1.222
  • LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • LFNG
  • lunatic fringe (Drosophila) homolog
  • lunatic fringe homolog (Drosophila)
  • Lunatic Fringe
  • O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • SCDO3
  • SCDO3EC 2.4.1.222

Background

Lunatic Fringe encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol LFNG
Uniprot