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Recombinant Human CXCL10/IP-10 Protein

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Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3. The ED50 for this effect is 0.03-0.18 μg/mL.
1 µg/lane of Recombinant Human CXCL10/IP-10/CRG-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CXCL10/IP-10 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED50 for this effect is 0.03‑0.18 µg/mL.
Source
E. coli-derived human CXCL10/IP-10/CRG-2 protein
Val22-Pro98, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL10
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
266-IP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL10/IP-10 Protein

  • C7
  • chemokine (C-X-C motif) ligand 10
  • CRG2
  • CRG-2
  • CXCL10
  • gIP-10
  • IFI10
  • INP10
  • IP-10
  • mob-1
  • SCYB10

Background

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta , TNF‑ alpha , IL-12 and viruses. Additional cell types that have been shown to express CXCL10 include activated T‑lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T‑lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al .(1996) J. Biol. Chem. 271:24286.

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Publications for CXCL10/IP-10/CRG-2 (266-IP)(55)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Tree Shrew, Virus.

We have publications tested in 6 applications: Binding Assay, Bioassay, ELISA (Standard), ELISA Standard, Enzyme Assay, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(39)
ELISA (Standard)
(12)
ELISA Standard
(1)
Enzyme Assay
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(40)
Mouse
(2)
Rat
(1)
N/A
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Tree Shrew
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using 266-IP Applications Species
Domínguez-Luis, MJ;Castro-Hernández, J;Santos-Concepción, S;Díaz-Martín, A;Arce-Franco, M;Pérez-González, N;Díaz, M;Castrillo, A;Salido, E;Machado, JD;Gumá, M;Corr, M;Díaz-González, F; Modulation of the K/BxN arthritis mouse model and the effector functions of human fibroblast-like synoviocytes by liver X receptors European journal of immunology 2024-08-15 [PMID: 39148175] (Bioassay, Human) Bioassay Human
Tabasum, S;Thapa, D;Giobbie-Hurder, A;Weirather, JL;Campisi, M;Schol, PJ;Li, X;Li, J;Yoon, CH;Manos, MP;Barbie, DA;Hodi, FS; EDIL3 as an angiogenic target of immune exclusion following checkpoint blockade Cancer immunology research 2023-09-20 [PMID: 37728484] (Bioassay, Human) Bioassay Human
LJ Pallett, L Swadling, M Diniz, AA Maini, M Schwabenla, AD Gasull, J Davies, S Kucykowicz, JK Skelton, N Thomas, NM Schmidt, OE Amin, US Gill, KA Stegmann, AR Burton, E Stephenson, G Reynolds, M Whelan, J Sanchez, R de Maeyer, C Thakker, K Suveizdyte, I Uddin, AM Ortega-Pri, C Grant, F Froghi, G Fusai, S Lens, S Pérez-Del-, W Al-Akkad, G Mazza, M Noursadegh, A Akbar, PTF Kennedy, BR Davidson, M Prinz, BM Chain, M Haniffa, DW Gilroy, M Dorner, B Bengsch, A Schurich, MK Maini Tissue CD14+CD8+ T cells reprogrammed by myeloid cells and modulated by LPS Nature, 2023-01-25;614(7947):334-342. 2023-01-25 [PMID: 36697826] (Bioassay, Human) Bioassay Human
R Lei, H Arain, M Obaid, N Sabhnani, C Mohan Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases Biosensors, 2022-09-14;12(9):. 2022-09-14 [PMID: 36140141] (Bioassay, Human) Bioassay Human
L Rijvers, J van Langel, L Bogers, MJ Melief, SC Koetzier, KM Blok, AF Wierenga-W, HE De Vries, J Rip, OB Corneth, RW Hendriks, R Grenninglo, U Boschert, J Smolders, MM van Luijn Human T-bet+ B cell development is associated with BTK activity and suppressed by evobrutinib JCI Insight, 2022-08-22;0(0):. 2022-08-22 [PMID: 35852869] (Bioassay, Human) Bioassay Human
ST Kim, Y Chu, M Misoi, ME Suarez-Alm, JH Tayar, H Lu, M Buni, J Kramer, E Rodriguez, Z Hussain, SS Neelapu, J Wang, AY Shah, NM Tannir, MT Campbell, DL Gibbons, T Cascone, C Lu, GR Blumensche, M Altan, B Lim, V Valero, ME Loghin, J Tu, SN Westin, A Naing, G Garcia-Man, N Abdel-Waha, HA Tawbi, P Hwu, ICG Oliva, MA Davies, SP Patel, J Zou, A Futreal, A Diab, L Wang, R Nurieva Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapy Nature Communications, 2022-04-12;13(1):1970. 2022-04-12 [PMID: 35413951] (Bioassay, Human) Bioassay Human
L Déry, G Charest, B Guérin, M Akbari, D Fortin Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors International Journal of Molecular Sciences, 2021-11-10;22(22):. 2021-11-10 [PMID: 34830041] (Bioassay, In Vivo, Human, Rat) Bioassay, In Vivo Human, Rat
M Kim, HY Choi, JW Woo, YR Chung, SY Park Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast Scientific Reports, 2021-09-09;11(1):18007. 2021-09-09 [PMID: 34504204] (Bioassay, Human) Bioassay Human
R Ravindran, C McReynolds, J Yang, BD Hammock, A Ikram, A Ali, A Bashir, T Zohra, WLW Chang, DJ Hartigan-O, HH Rashidi, IH Khan Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses PLoS ONE, 2021-07-09;16(7):e0254367. 2021-07-09 [PMID: 34242356] (ELISA Standard, Human) ELISA Standard Human
C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma Cancers, 2021-03-16;13(6):. 2021-03-16 [PMID: 33809574] (Human) Human
Show All 55 Publications.

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Bioinformatics

Gene Symbol CXCL10
Uniprot