Home » CCL16/HCC-4/LEC » CCL16/HCC-4/LEC Proteins » Recombinant Human CCL16/HCC-4 Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Human CCL16/HCC-4 Protein

 (6 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for CCL16/HCC-4/LEC (802-HC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
802-HC-025/CF
Carrier Free
802-HC-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human CCL16/HCC-4 Protein Summary

Details of Functionality
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. Hedrick, J. et al. (1998) Blood 91:4242. The ED50 for this effect is 0.5‑2 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.15‑0.75 µg/mL.
Source
E. coli-derived human CCL16/HCC-4 protein
Gln24-Gln120
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL16
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
802-HC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL16/HCC-4 Protein

  • C-C motif chemokine 16
  • CCL16
  • chemokine (C-C motif) ligand 16
  • Chemokine CC-4
  • Chemokine LEC
  • CKb12
  • HCC4
  • HCC-4
  • HCC-4Liver-expressed chemokine
  • LCC-1Lymphocyte and monocyte chemoattractant
  • LEC
  • liver CC chemokine-1
  • LMC
  • LMCSmall-inducible cytokine A16
  • monotactin-1
  • Mtn-1
  • NCC-4
  • NCC4IL-10-inducible chemokine
  • NCC-4ILINCK
  • new CC chemokine 4
  • SCYA16MGC117051
  • SCYL4
  • small inducible cytokine subfamily A (Cys-Cys), member 16

Background

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.

Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

  1. Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
  2. Hedrick, J. et al. (1998) Blood 91:4242.
  3. Youn, B-S. et al. (1998) BBRC 247:217.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA
VEGFR3/Flt-4
Antibody [Unconjugate
AF743
Flow Cytometry VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
(3 Reviews)
(137 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
VEGF-C [HRP]
DVEC00
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
(53 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(674 Publications)
Species: Hu
Applications: ELISA
CCL15/MIP-1 delta
Antibody (52130) [Unc ...
MAB363
Neutralization CCL15/MIP-1 delta Antibody (52130) [Unconjugated]
(3 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
CCR1 Antibody
(53504) [Unconjugated
MAB145
Flow Cytometry CCR1 Antibody (53504) [Unconjugated]
(1 Review)
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
Podoplanin
Antibody [Unconjugate
AF3244
Western Blot Podoplanin Antibody [Unconjugated]
Immunohistochemistry Podoplanin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Podoplanin Antibody [Unconjugated]
(1 Review)
(73 Publications)
Species: Mu
Applications: IHC, Simple Western, WB
LYVE-1 Antibody
[Unconjugated]
AF2125
Immunohistochemistry LYVE-1 Antibody [Unconjugated]
Western Blot LYVE-1 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence LYVE-1 Antibody [Unconjugated]
(1 Review)
(133 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(251 Publications)
Species: Hu
Applications: ELISA
ATP7b Antibody -
BSA Free
NB100-360
Immunocytochemistry/ Immunofluorescence ATP7b Antibody - BSA Free
Western Blot ATP7b Antibody - BSA Free
Immunohistochemistry ATP7b Antibody - BSA Free
(1 Review)
(22 Publications)
Species: Hu, Mu, Rt
Applications: IB, ICC/IF, IHC, IHC-P, IP, WB
CCL14/HCC-1/HCC-3
Antibody (256409)
MAB3241
Neutralization CCL14/HCC-1/HCC-3 Antibody (256409)
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(370 Publications)
Species: Hu
Applications: BA
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Ceruloplasmin
Antibody (6C3K9)
NBP3-15868
Immunohistochemistry-Paraffin Ceruloplasmin Antibody (6C3K9)
Immunohistochemistry-Paraffin Ceruloplasmin Antibody (6C3K9)
Immunohistochemistry Ceruloplasmin Antibody (6C3K9)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(531 Publications)
Species: Hu
Applications: BA
PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/ Immunofluorescence PCNA Antibody (PC10)
Western Blot PCNA Antibody (PC10)
Flow Cytometry PCNA Antibody (PC10)
(5 Reviews)
(45 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
CCR8 Antibody
(191704) [Unconjugate
MAB1429
Flow Cytometry CCR8 Antibody (191704) [Unconjugated]
Neutralization CCR8 Antibody (191704) [Unconjugated]
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, Neut

Publications for CCL16/HCC-4/LEC (802-HC)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Virus.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Virus
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 802-HC Applications Species
V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982140] (Bioassay, Human) Bioassay Human
Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533380] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Jang SW, Kim YS, Kim YR, Sung HJ, Ko J Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1. J. Biol. Chem., 2007-02-12;282(15):11092-100. 2007-02-12 [PMID: 17296613] (Bioassay, Human) Bioassay Human
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus) Binding Assay, Bioassay Virus
Cappello P, Caorsi C, Bosticardo M CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity. J. Leukoc. Biol., 2003-10-02;75(1):135-42. 2003-10-02 [PMID: 14525962] (Bioassay, Mouse) Bioassay Mouse

Reviews for CCL16/HCC-4/LEC (802-HC) (0)

There are no reviews for CCL16/HCC-4/LEC (802-HC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CCL16/HCC-4/LEC (802-HC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCL16/HCC-4/LEC Products

Blogs on CCL16/HCC-4/LEC

There are no specific blogs for CCL16/HCC-4/LEC, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CCL16/HCC-4 Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL16
Uniprot