Recombinant Human BAFF/BLyS/TNFSF13B Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Surface Plasmon Resonance (SPR.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using 2149-BF/CF	Applications	Species
J Kim, SMT Islam, F Qiao, AK Singh, M Khan, J Won, I Singh Regulation of B cell functions by S-nitrosoglutathione in the EAE model Redox Biology, 2021-06-23;45(0):102053. 2021-06-23 [PMID: 34175668] (Bioassay, Mouse)	Bioassay	Mouse
M Mulazzani, M Huber, S Borchard, S Langer, B Angele, E Schuh, E Meinl, M Dreyling, T Birnbaum, A Straube, U Koedel, L von Baumga APRIL and BAFF: novel biomarkers for central nervous system lymphoma J Hematol Oncol, 2019-10-15;12(1):102. 2019-10-15 [PMID: 31615554] (Bioassay, Human)	Bioassay	Human
R Wilson, M Makuch, AK Kienzler, J Varley, J Taylor, M Woodhall, J Palace, MI Leite, P Waters, SR Irani Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica Brain, 2018-04-01;141(4):1063-1074. 2018-04-01 [PMID: 29447335] (Bioassay, Human)	Bioassay	Human
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human)	Surface Plasmon Resonance (SPR	Human
J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome J. Allergy Clin. Immunol., 2018-01-31;0(0):. 2018-01-31 [PMID: 29391254] (Flow Cytometry, Human)	Flow Cytometry	Human
HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018-01-12;9(1):179. 2018-01-12 [PMID: 29330524] (Bioassay, Human)	Bioassay	Human
D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL Mol. Cancer Ther., 2017-08-23;0(0):. 2017-08-23 [PMID: 28835384] (Bioassay, Human)	Bioassay	Human
VJ Sindhava, MA Oropallo, K Moody, M Naradikian, LE Higdon, L Zhou, A Myles, N Green, K Nündel, W Stohl, AM Schmidt, W Cao, S Dorta-Estr, T Kambayashi, A Marshak-Ro, MP Cancro A TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens J. Clin. Invest, 2017-03-27;0(0):. 2017-03-27 [PMID: 28346226] (Bioassay, Human)	Bioassay	Human
Mamara , Antigoni, Germenis , Anastasi, Kompoti , Maria, Palassopoulou , Maria, Mandala , Eudokia, Banti , Anastasi, Giannakoulas , Nikolaos, Speletas , Matthaio TACI expression and signaling in chronic lymphocytic leukemia. J Immunol Res, 2015-04-09;2015(0):478753. 2015-04-09 [PMID: 25950010] (Bioassay, Human)	Bioassay	Human
Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma. Cancer Res, 2014-09-30;74(24):7418-29. 2014-09-30 [PMID: 25269480] (Bioassay, Human)	Bioassay	Human
Caro-Maldonado , Alfredo, Wang , Ruoning, Nichols , Amanda G, Kuraoka , Masayuki, Milasta , Sandra, Sun , Lillian, Gavin , Amanda L, Abel , E Dale, Kelsoe , Garnett, Green , Douglas, Rathmell , Jeffrey Metabolic reprogramming is required for antibody production that is suppressed in anergic but exaggerated in chronically BAFF-exposed B cells. J Immunol, 2014-03-10;192(8):3626-36. 2014-03-10 [PMID: 24616478] (Bioassay, Human)	Bioassay	Human
Deknuydt F, Nordstrom T, Riesbeck K Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles. J Leukoc Biol, 2014-02-18;95(6):983-91. 2014-02-18 [PMID: 24550522] (Bioassay, Human)	Bioassay	Human
Sahaf B, Yang Y, Arai S, Herzenberg L, Herzenberg L, Miklos D H-Y antigen-binding B cells develop in male recipients of female hematopoietic cells and associate with chronic graft vs. host disease. Proc Natl Acad Sci U S A, 2013-02-04;110(8):3005-10. 2013-02-04 [PMID: 23382226] (Bioassay, Human)	Bioassay	Human
Overexpression of the autoimmunity-associated phosphatase PTPN22 promotes survival of antigen-stimulated CLL cells by selectively activating AKT. Blood, 2012-05-08;119(26):6278-87. 2012-05-08 [PMID: 22569400] (Bioassay, Human)	Bioassay	Human
Zhu J, Sun L, Lin S BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. J. Exp. Clin. Cancer Res., 2012-03-31;31(0):31. 2012-03-31 [PMID: 22463935] (Bioassay, Human)	Bioassay	Human
Bloom D, Chang Z, Pauly K BAFF is increased in renal transplant patients following treatment with alemtuzumab. Am. J. Transplant., 2009-06-12;9(8):1835-45. 2009-06-12 [PMID: 19522878] (Bioassay, Human)	Bioassay	Human
Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model. Clin. Cancer Res., 2007-10-01;13(19):5903-9. 2007-10-01 [PMID: 17908986] (Bioassay, Human)	Bioassay	Human
Kaur K, Chowdhury S, Greenspan NS, Schreiber JR Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates. Blood, 2007-07-18;110(8):2948-54. 2007-07-18 [PMID: 17634409] (Bioassay, Human)	Bioassay	Human
Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J. Immunol., 2007-03-01;178(5):2872-82. 2007-03-01 [PMID: 17312131] (Bioassay, Human)	Bioassay	Human
Show All 19 Publications. Collapse Publications.

Array 2149-BF/CF

• BAFF/BLyS/TNFSF13B Antibodies
• BAFF/BLyS/TNFSF13B Antibody Pair
• BAFF/BLyS/TNFSF13B ELISA Kit
• BAFF/BLyS/TNFSF13B Lysate
• BAFF/BLyS/TNFSF13B Proteins
• BAFF/BLyS/TNFSF13B ELISAs
• BAFF/BLyS/TNFSF13B Proteins and Enzymes