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Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

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When Recombinant HumanAICL/CLEC-2B Fc Chimera (Catalog #9059-CL) is coated at 2 µg/mL, Recombinant Human Galectin-1(Catalog # 1152-GA) binds with a typical ED50 of0.6-3 µg/mL.
When Recombinant HumanAICL/CLEC-2B Fc Chimera (Catalog #9059-CL) is coated at 2 µg/mL, Recombinant Human Galectin-1(Catalog # 1152-GA) binds with a typical ED50 of0.6-3 µg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human AICL/CLEC-2B Fc Chimera is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces 50% optimal binding response is 0.6-3 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human AICL/CLEC-2B
(Lys26-His149) with a Met79Thr substitution
Accession # Q92478
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CLEC2B
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
48-65 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

  • Activation-induced C-type lectin
  • AICL
  • AICLC-type lectin superfamily member 2
  • CLEC2B
  • CLEC-2B
  • CLECSF2
  • CLECSF2IFN-alpha-2b-inducing-related protein 1
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced)
  • C-type lectin domain family 2 member B
  • C-type lectin domain family 2, member B
  • HP10085
  • IFN-alpha2b-inducing related protein 1
  • IFNRG1

Background

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).
  1. Li, Y. et al. (2014) Front. Immunol. 5:123.
  2. Hamann, J. et al. (1997) Immunogenetics 45:295.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Klimosch, S.N. et al. (2013) Blood 122:2380.

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Recombinant Human
AICL/CLEC-2B Fc Chime ...
9059-CL
Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF
Species: Hu
Applications: Bioactivity

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Bioinformatics

Gene Symbol CLEC2B
Uniprot