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Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhActivin RIB/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhCripto with a linear range of 0.8-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Activin RIB/ALK-4 protein
Human Activin RIB
(Leu32 - Glu126)
Accession # AAA60556		 IEGRMD Human IgG1
(Pro100 - Lys330) 		6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ACVR1B
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
37.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-50 kDa, reducing conditions
Publications
Read Publications using
808-AR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF

  • activin A receptor, type IB
  • Activin receptor type IB
  • Activin receptor-like kinase 4
  • Activin RIB
  • ActivinRIB
  • ACTRIB
  • ACVR1B
  • ACVRLK4ACTR-IB
  • ALK4
  • ALK-4
  • ALK4activin A receptor, type II-like kinase 4
  • EC 2.7.11
  • EC 2.7.11.30
  • Serine/threonine-protein kinase receptor R2
  • SKR2activin receptor type-1B

Background

Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25% - 35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7 - 9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).

  1. Chen, Y.- G. et al. (2006) Exp. Biol. Med. 231:534. 
  2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066. 
  3. Tsuchida, K. et al. (1995) Endocrinology 136:5493. 
  4. ten Dijke, P. et al. (1993) Oncogene 8:2879. 
  5. Xu, J. et al. (1994) Proc. Natl. Acad. Sci. 91:7957. 
  6. Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066. 
  7. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
  8. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
  9. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  10. Lewis, K.A. et al. (2000) Nature 404:411.
  11. Gu, Z. et al. (1998) Genes Dev. 12:844.
  12. Chen, Y. et al. (2004) Dev. Biol. 268:280.

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Publications for Activin RIB/ALK-4 (808-AR)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance.


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Bioassay
(2)
Surface Plasmon Resonance
(1)
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Human
(3)
Mouse
(1)
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Bioinformatics

Gene Symbol ACVR1B
Uniprot