Recombinant Canine LIF Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

LIF (leukemia inhibitory factor) is a 32-62 kDa, variably glycosylated, monomeric member of the IL-6 family of helical cytokines (1-4). LIF is widely expressed and highly pleiotropic. It is important for many physiological processes including embryo implantation and development, hematopoiesis, bone metabolism, and inflammation (5). Canine LIF is synthesized as a 202 amino acid (aa) precursor protein with a 22 aa signal sequence that can be alternately spliced to produce a secreted (LIF-D), extracellular matrix-associated (LIF-M), or intracellular (LIF-T) isoform (6). LIF-D and LIF-M have identical 180 aa mature sequences (6). Mature canine LIF shares 91% sequence identity with human LIF, 80% sequence identity with mouse and rat LIF, and 91% identity with bovine and porcine LIF. LIF induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (LIF R) and a 130 kDa signal transducing subunit (gp130). The gp130 subunit does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. LIF has been shown to support embryo implantation and to promote immunosuppression during pregnancy (7, 8). During development, it regulates cell proliferation and differentiation. However, its ability to favor or oppose either of these events is cell and context dependent (4, 5). LIF is important in the proliferation and maintenance of canine embryonic stem cells and induced pluripotent stem cells (9-11). LIF is up-regulated in activated CD4⁺ T cells, promotes regulatory T cell differentiation, and inhibits Th17 cell function (2, 5, 12). In the brain, it is up-regulated by neuronal injury and promotes neuron survival and oligodendrocyte myelination (2, 4, 13). It is produced by the adrenal cortex and enhances adrenal production of cortisol and aldosterone (14). Tumor cell-derived LIF has been shown to promote the differentiation of monocytes into tumor-associated macrophages (15, 16). LIF also promotes endometrial remodeling and the differentiation of adipocytes and cardiac smooth muscle cells (2, 4, 5).

Array 8600-LF

• LIF Antibodies
• LIF ELISA Kits
• LIF Lysate
• LIF Proteins
• LIF ELISAs
• LIF Proteins and Enzymes