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Recombinant Canine IL-4 Protein, CF

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Canine IL-4 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3‑18 ng/mL.
Source
E. coli-derived canine IL-4 protein
His25-His132, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
754-CL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • IL4E12
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8-11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Khatlani, T. et al. (1999) J. Vet. Med. Sci. 61:967.
  4. van der Kaaij, S.Y. et al. (1999) Immunogenetics 49:142.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (754-CL/CF)(20)

We have publications tested in 2 confirmed species: Mouse, Canine.

We have publications tested in 4 applications: Bioassay, Cell Culture, Control, ELISA (Standard).


Filter By Application
Bioassay
(12)
Cell Culture
(1)
Control
(1)
ELISA (Standard)
(6)
All Applications
Filter By Species
Mouse
(1)
Canine
(16)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 754-CL/CF Applications Species
M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox Immortalised canine buccal epithelial cells&#039; CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol Veterinary research, 2022-09-13;53(1):72. 2022-09-13 [PMID: 36100942] (Bioassay, Canine) Bioassay Canine
CO Santos, SF Costa, FS Souza, JMF Mendes, CGM de Pinheir, DRM Moreira, LK Silva, VMF de Lima, GG de Sá Oliv Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum PLoS ONE, 2021-01-19;16(1):e0239171. 2021-01-19 [PMID: 33465107] (Bioassay, Mouse) Bioassay Mouse
LW Stranahan, SP Chaki, DG Garcia-Gon, OH Khalaf, AM Arenas-Gam Evaluation of the Efficacy of the Brucella canis RM6/66 deltavjbR Vaccine Candidate for Protection against B. canis Infection in Mice mSphere, 2020-05-20;5(3):. 2020-05-20 [PMID: 32434839] (Bioassay, Canine) Bioassay Canine
N Milhau, E Almouazen, S Bouteille, I Hellel-Bou, S Azzouz-Maa, U Benavides, AF Petavy, T Marchal In vitro evaluations on canine monocyte-derived dendritic cells of a nanoparticles delivery system for vaccine antigen against Echinococcus granulosus PLoS ONE, 2020-02-26;15(2):e0229121. 2020-02-26 [PMID: 32101539] (Cell Culture, Canine) Cell Culture Canine
EM van Helvoo, J Popov-Cele, N Eijkelkamp, K Coeleveld, MA Tryfonidou, CD Wijne, CE Hack, FPJG Lafeber, SC Mastbergen Canine IL4-10 fusion protein provides disease modifying activity in a canine model of OA; an exploratory study PLoS ONE, 2019-07-11;14(7):e0219587. 2019-07-11 [PMID: 31295306] (Control, Canine) Control Canine
J Thalmensi, E Pliquet, C Liard, G Chamel, C Kreuz, T Bestetti, M Escande, A Kostrzak, AS Pailhes-Ji, E Bourges, M Julithe, L Bourre, O Keravel, P Clayette, T Huet, S Wain-Hobso, P Langlade-D A DNA telomerase vaccine for canine cancer immunotherapy Oncotarget, 2019-05-21;10(36):3361-3372. 2019-05-21 [PMID: 31164958] (Bioassay, Canine) Bioassay Canine
M Pujol, C Borie, M Montoya, A Ferreira, R Vernal Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation Comp. Immunol. Microbiol. Infect. Dis., 2018-11-30;62(0):68-75. 2018-11-30 [PMID: 30711049] (Bioassay, Canine) Bioassay Canine
V Konduri, MM Halpert, YC Baig, R Coronado, JR Rodgers, JM Levitt, B Cerroni, S Piscoya, N Wilson, L DiBernardi, Z Omarbekov, L Seelhoff, V Ravi, L Douglass, WK Decker Dendritic cell vaccination plus low-dose doxorubicin for the treatment of spontaneous canine hemangiosarcoma Cancer Gene Ther., 2019-01-23;0(0):. 2019-01-23 [PMID: 30670791] (Bioassay, Canine) Bioassay Canine
AJ Hernández-, MA Franco-Mol, EE Coronado-C, P Zapata-Ben, EM Gamboa, Y Ramos-Zaya, SE Santana-Kr, C Rodríguez- Immunogenic potential of three transmissible venereal tumor cell lysates to prime canine-dendritic cells for cancer immunotherapy Res. Vet. Sci., 2018-10-05;121(0):23-30. 2018-10-05 [PMID: 30316013] (Bioassay, Canine) Bioassay Canine
F Heinrich, A Lehmbecker, BB Raddatz, K Kegler, A Tipold, VM Stein, A Kalkuhl, U Deschl, W Baumgärtne, R Ulrich, I Spitzbarth Morphologic, phenotypic, and transcriptomic characterization of classically and alternatively activated canine blood-derived macrophages in vitro PLoS ONE, 2017-08-17;12(8):e0183572. 2017-08-17 [PMID: 28817687] (Bioassay, Canine) Bioassay Canine
Show All 20 Publications.

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Bioinformatics

Gene Symbol IL4
Uniprot