Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse RAR gamma/NR1B3 in samples. No significant cross-reactivity or interference between Mouse RAR gamma/NR1B3 and analogues was observed. |
Standard Curve Range |
78.13 - 5000 pg/mL |
Sensitivity |
46.88 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
4.16% |
Intra-Assay |
4.6% |
Spike Recovery |
86 - 108% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
RARG |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric)
Background
Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters. Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition. While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrett's tissues compared with normal tissue.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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