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Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric)

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ELISA: Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) [NBP3-00454] - Samples were spiked with high concentrations of Mouse RAR gamma/NR1B3 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) [NBP3-00454] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse RAR gamma/NR1B3 in samples. No significant cross-reactivity or interference between Mouse RAR gamma/NR1B3 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.16%
Intra-Assay
4.6%
Spike Recovery
86 - 108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
RARG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric)

  • NR1B3
  • NR1B3RARC
  • Nuclear receptor subfamily 1 group B member 3
  • RAR gamma
  • RARC
  • RARG
  • RAR-gamma
  • retinoic acid receptor gamma
  • retinoic acid receptor, gamma

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters. Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition. While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrett's tissues compared with normal tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RARG