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RAPGEF5 Antibody (2E4)

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Western Blot: RAPGEF5 Antibody (2E4) [H00009771-M09] - Analysis of RAPGEF5 expression in Hela S3 NE (Cat # L013V3).
Western Blot: RAPGEF5 Antibody (2E4) [H00009771-M09] - Analysis of RAPGEF5 expression in Hela S3 NE (Cat # L013V3).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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RAPGEF5 Antibody (2E4) Summary

Immunogen
RAPGEF5 (AAH39203, 1 a.a. ~ 444 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSSRLRVFDPHLERKDSAAALSDRELPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDDSVDSLLSDRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTETEEIFCHVYITEHSYVSVKAKVSSIAQEILLCSQLGKRVQLVKKFIKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFRKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFVPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEHQELKSYVNHLYVIDSQQALFELSHRIEPRV
Specificity
RAPGEF5 - Rap guanine nucleotide exchange factor (GEF) 5 (2E4)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAPGEF5
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAPGEF5 Antibody (2E4)

  • GFRREPAC
  • Guanine nucleotide exchange factor for Rap1
  • KIAA0277M-Ras-regulated GEF
  • M-Ras-regulated Rap GEF
  • MRGEF
  • MR-GEFrap guanine nucleotide exchange factor 5
  • Rap guanine nucleotide exchange factor (GEF) 5
  • Related to Epac
  • Repac

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00009771-M09
RAPGEF5 Antibody (2E4)
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Applications: WB, ELISA

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Bioinformatics

Gene Symbol RAPGEF5
Entrez
Uniprot